Table 1.
Growth parameters at day 21 of life in control and ERA-treated male and female rats.
Fig 1.
Number of total glomeruli obtained by the maceration method. ERA: Endothelin receptor antagonist. Values are mean ± SEM (n = 10). Data were analyzed using two way ANOVA followed by Bonferroni posttest. Two-way ANOVA showed a statistically significant interaction (p < 0.05) between the effects of ERA treatment and sex. *p<0.05 vs control males.
Table 2.
Morphometric parameters in juxtamedullary and cortical renal tissue.
Fig 2.
A: Proteinuria (mg/24 h/100g). B: Creatinine Clearance (ml/min/100g).
ERA: Endothelin receptor antagonist. Values are mean ± SEM. Data were analyzed using two way ANOVA followed by Bonferroni posttest.
Table 3.
Renal functional parameters.
Fig 3.
Histological structure of the renal cortex.
Representative images of Hematoxylin-eosin staining of the renal cortex. A = control males; B = ERA-treated males; C = control females; D = ERA-treated females. ERA: Endothelin receptor antagonist. Total magnification: 400x.
Fig 4.
Sirius red staining of the renal cortex.
Representative images of Sirius red staining of the renal cortex; CA: cortical area; JA: juxtamedullary area. A = control males (CA); B = ERA-treated males (CA); C = control females (CA); D = ERA-treated females (CA); E = control males (JA); F = ERA-treated males (JA); G = control females (JA); H = ERA-treated females (JA). ERA: Endothelin receptor antagonist. Total magnification: 400x.
Fig 5.
Masson´s trichrome staining of the renal cortex.
Representative images of Masson´s trichrome staining of the renal cortex; CA: cortical area; JA: juxtamedullary area. A = control males (CA); B = ERA-treated males (CA); C = control females (CA); D = ERA-treated females (CA); E = control males (JA); F = ERA-treated males (JA); G = control females (JA); H = ERA-treated females (JA). ERA: Endothelin receptor antagonist. Total magnification: 400x.
Fig 6.
α-SMA immunohistochemistry of the renal cortex.
Representative images of α-SMA immunohistochemistry of the renal cortex; CA: cortical area; JA: juxtamedullary area. A = control males (CA); B = ERA-treated males (CA); C = control females (CA); D = ERA-treated females (CA); E = control males (JA); F = ERA-treated males (JA); G = control females (JA); H = ERA-treated females (JA). ERA: Endothelin receptor antagonist. Total magnification: 400x.
Table 4.
Renal histochemical evaluation by Sirius red and Masson´s thrichrome.