Table 1.
Sprouting date of ZC108 and LJ43 cultivated in two areas in spring of 2013 and 2014.
Fig 1.
Growth performance and leaf morphology of LJ43 and ZC108 grown in the field.
(A, B) Growth performance of ZC108 (A) and LJ43 (B) in the field in spring. (C) Mature leaf morphology of LJ43 and ZC108, bar = 1 cm. (D) Length:width ratio in mature leaves of LJ43 and ZC108. Data shown as the mean ±SD (n = 35). **P < 0.01 vs LJ43.
Fig 2.
Altered disease resistance of tea leaves to AD in ZC108.
(A, B) Leaf symptoms of LJ43 (A) and ZC108 (B) after suffering from AD in the field. (C, D) Differential disease resistance of tea leaves to Colletotrichum camelliae (1×105 ml-1) infection in LJ43 and ZC108. Two strains of Colletotrichum camelliae pathogens were used. Colletotrichum camelliae-1 and -2 were isolated from diseased tea plant leaves in Shaanxi and Zhejiang, respectively. Photos were taken on 3, 7 and 14 days post inoculation.
Fig 3.
Differential expression analyses in ZC108 and LJ43.
(A) Number of significantly differentially expressed genes (P < 0.05, fold change (FC) > 2) in ZC108 compared with LJ43. (B) Significantly (P < 0.05, FDR < 0.05) enriched pathways (based on KEGG) among the 2,453 differentially expressed genes. (C) GO classification of the 2,453 differentially expressed genes.
Table 2.
Differentially expressed genes involved in phenylpropanoid biosynthesis (FC > 2, P < 0.05).
Table 3.
Differentially expressed genes related to plant hormone biosynthesis and signaling pathways (FC > 2, P < 0.05).
Table 4.
Differentially expressed genes mapped to the plant-pathogen interaction pathway (KO04626) in KEGG (FC > 2, P < 0.05).
Table 5.
Classification of differentially expressed genes encoding transcription factors.
Fig 4.
Validation and expression analysis of 27 genes in response to Colletotrichum camelliae infection in leaves of LJ43 and ZC108.
Gene expression analysis were examined by qRT-PCR using cDNA from leaves of three-year-old LJ43 and ZC108 after inoculated by Colletotrichum camelliae for 0, 24 and 72 h. All of the qRT-PCR values were expressed relative to the expression level of LJ43-0h (control-set to 1.0). The data of ZC108 from microarray was expressed relative to the transcript abundance of LJ43 from microarray (control-set to 1.0). The color scale represents log2 expression values and the expression levels presented in heatmap were log2-based. All data are shown as the mean ± SD (n = 3).