Table 1.
Average carbonate chemistry with standard deviation in brackets from July 2011 to July 2015 for the long-term ocean acidification experiment with Tanner Crab (Chionoecetes bairdi).
pCO2, partial pressure of carbon dioxide in μatm; HCO3-, bicarbonate ion in μmol kg-1; CO3-2. bicarbonate ion in μmol kg-1; DIC, dissolved inorganic carbon in μmol kg-1;ALK, alkalinity in μmol kg-1; ΩAr, calculated aragonite saturation state; ΩCa, calculated calcite saturation state.
Fig 1.
Forward Scatter (FSC-H) versus Side Scatter (SSC-H) of hemolymph from Chionoecetes bairdi, Tanner Crab.
Table 2.
The percent change of the mean FSC-H and SSC-H for both hyalinocyte cells (HC) and semi-granular+granular cells (SGC+GC) relative to a sample of hemocytes without the potassium buffer, nigericin, or SNARF from Tanner crab (Chionoecetes bairdi).
The potassium and nigericin concentrations listed are the final concentrations in the test tube after all the reagents and hemolymph were added.
Fig 2.
The calibration profile of Carboxy SNARF® for Chionoecetes bairdi, Tanner Crab.
The slope of the line was used to calculate pHi of semi-granular+ granular cells and hyalinocyte cells.
Fig 3.
Live and dead semi-granular+granular cells (SGC+GC) and hyalinocyte cells (HC) for Chionoecetes bairdi, Tanner Crab.
The upper region contains the semi-granular and granular cells, while the lower region contains the hyalinocytes. The error bars for the dead cells are negative, while the error bar for the live cells is positive. Different homogenous groups are labeled for only the dead cells since there was no significant difference in the number of total cells.
Fig 4.
The percentage of phagocytic hemocytes in Chionoecetes bairdi, Tanner Crab, from each pH treatment.
The error bars represent the standard error and a different letter indicates a significant difference between treatments.
Fig 5.
Hemolymph pH (pHe) and intracellular pH (pHi) for semi-granular+granular cells (SGC+GC) and hyalinocyte cells (HC) from Chionoecetes bairdi, Tanner crab.
The error bars represent the standard error and letters indicate significant differences between treatments.
Fig 6.
Relationship between hemocyte function in and parameters measured in the adult Chionoecetes bairdi, Tanner Crab.
The hemocyte function that showed an relationship include SGC+GC pHi, phagocytosis, and % dead hemocytes relative to the adult parameters for number of eggs in second hatch, % viable/non-viable eggs, and calcium concentration.