Table 1.
Descriptive data for 14 California mountain lions (Puma concolor) tested between January 1995 and August 2003.
Table 2.
Descriptive data for 19 California bobcats (Lynx rufus) tested between May 1995 and September 1998.
Table 3.
Phenotypic characteristics of Bartonella species isolated from domestic and wild felids, including the new subspecies B. koehlerae subsp. boulouisii and B. koehlerae subsp. bothieri.
Table 4.
PCR-RFLP comparison of the citrate synthase gene (gltA) of two novel Bartonella subspecies isolated from California mountain lions and bobcats, with Bartonella species isolated from domestic cats: B. henselae genotype I (Bh I) and genotype II (Bh II), B. koehlerae (Bk), and B. clarridgeiae (Bc).
Table 5.
PCR-RFLP comparison of the 16S rRNA gene, ribC and internal transcribed spacer region (ITS) of two novel Bartonella subspecies isolated from California mountain lions and bobcats, with Bartonella species isolated from domestic cats: B. henselae genotype I (Bh I) and genotype II (Bh II), and B. koehlerae (Bk).
Fig 1.
PCR-RFLP of a gltA gene fragment using the restriction endonuclease AciI.
Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B. henselae Type I; Lane 15, B. henselae Type II; Lane 16, B. clarridgeiae; Lane 17, B. koehlerae; Lane18, B. bovis (“weissii” isolate).
Fig 2.
PFGE analysis of SmaI-digested genomic DNA from selected Bartonella species.
Lane A1, molecular size standards (48.5 to 970 kbp); lane A2, B. henselae (type I Houston-I strain; ATCC 49882); lane A3, B. clarridgeiae (ATCC 51734); lane A4, B. koehlerae (ATCC 700693) (from reference 41); lane B1, B. henselae (type II Marseille strain; U4 U.C. Davis); lane B2, Mt Lion strain L42-94; lane B3, Mt Lion L27-96; lane B4, Bobcat strain L-17-96 (this study).
Table 6.
GenBank Accession Numbers.
Table 7.
DNA relatedness of L-42-94 mountain lion isolate with other Bartonella species.
Fig 3.
Phylogenetic relationships based on the sequence of a citrate synthase (gltA) gene fragment amplified from Bartonella species isolated from selected mountain lions and bobcats, compared with known species of the genus Bartonella.
The phylogenetic tree was constructed from 270 bp sequences, inferred using the Neighbor-Joining method, and 1000 replicates in the bootstrap test. Scale bar indicates 5 substitutions per nucleotide position.
Fig 4.
Phylogenetic relationships based on the sequence of an rpoB gene fragment amplified from Bartonella species isolated from selected mountain lions and bobcats, compared with known species of the genus Bartonella.
The phylogenetic tree was constructed from 771 bp sequences, inferred using the Neighbor-Joining method, and 1000 replicates in the bootstrap test. Scale bar indicates 5 substitutions per nucleotide position.
Fig 5.
Phylogenetic relationships based on the concatenated sequences of gltA, ftsZ, and rpoB gene fragments and the 16S-23S ITS region of the four mountain lion isolates and seven bobcat isolates, compared with known species of the genus Bartonella.
The phylogenetic tree was constructed from 2163 bp sequences, inferred using the Neighbor-Joining method, and 1000 replicates in the bootstrap test. Scale bar indicates 20 substitutions per nucleotide position.