Table 1.
Summary of Illumina transcriptome sequencing for Athetis dissimilis.
Table 2.
Summary of de novo assembly of the Athetis dissimilis transcriptome.
Fig 1.
Characteristics of homology search for Athetis dissimilis unigenes.
The number of unigenes matching the top ten species using BlastX in the Nr database is indicated in square brackets
Table 3.
Functional annotation of the Athetis dissimilis transcriptome.
Fig 2.
Functional annotation of assembled sequences based on gene ontology (GO) categorization.
GO analysis was performed at the level for three main categories (cellular component, molecular function, and biological process)
Fig 3.
Clusters of orthologous groups (COG) classification.
In total, 5967 unigenes with Nr hits were grouped into 25 COG classifications
Fig 4.
Distribution of each KEGG pathway number against the KEGG database.
Each color represents a KEGG pathway. The top 13 KEGG pathways are indicated. The number of unigenes mapped in each pathway is indicated in square brackets. The abbreviations represent the pathways as follows: Ko03010: Ribosome; ko04141: Protein processing in endoplasmic reticulum; ko00230: Purine metabolism; ko03013: RNA transport; ko00190: Oxidative phosphorylation; ko03040: Spliceosome; ko04120: Ubiquitin mediated proteolysis; ko04142: Lysosome; ko00240: Pyrimidine metabolism; ko04146: Peroxisome; ko04144: Endocytosis; ko03008: Ribosome biogenesis in eukaryotes.
Fig 5.
Neighbor-joining tree of candidate olfactory receptor (OR) and pheromone receptor (PR) genes from Athetis dissimilis and other Lepidoptera.
Unrooted tree was constructed using the BioNJ algorithm in Seaview v.4, which was made based on a sequence alignment using ClustalX 1.83. ORCO and PR genes are labeled in blue and red, respectively. Adis, Athetis dissimilis; Dple, Danaus plexippus; Cpom, Cydia pomonella; Bmor, Bombyx mori; Harm, Helicoverpa armigera.
Table 4.
Unigenes of candidate olfactory receptors.
Fig 6.
Neighbor-joining tree of candidate gustatory receptor (GR) genes from Athetis dissimilis and other insects.
Unrooted tree was constructed using the BioNJ algorithm in Seaview v.4, which was made based on a sequence alignment using ClustalX 1.83. The red and blue indicate sugar and CO2 receptor genes, respectively. Adis, Athetis dissimilis; Dmel, Drosophila melanogaster; Bmor, Bombyx mori; Hass, Helicoverpa assulta.
Table 5.
Unigenes of candidate gustatory receptors.
Fig 7.
Neighbor-joining tree of candidate ionotropic receptor (IR) genes from Athetis dissimilis and other insects.
Unrooted tree was constructed using the BioNJ algorithm in Seaview v.4, which was made based on a sequence alignment using ClustalX 1.83. Adis, Athetis dissimilis; Slit, Spodoptera littoralis; Hass, Helicoverpa assulta; Tmol, Tenebrio molitor; Dpon, Dendroctonus ponderosae; Ityp, Ips typographus.
Table 6.
Unigenes of candidate ionotropic receptors.