Fig 1.
Schematic map of the Alligator sinensis immunoglobulin light chain gene loci.
(A) Schematic map of the Alligator sinensis immunoglobulin light chain λ gene loci. (B) Schematic map of the Alligator sinensis immunoglobulin light chain κ gene loci. V: variable gene segments; J: joining gene segments; C: constant region gene; pseudo-variable gene segments were not be shown in the figure.
Fig 2.
Southern blotting detection of the Alligator sinensis Ig light chain C gene segments.
Genomic DNA was digested with restriction endonucleases, which are indicated above each lane, and hybridized with probes for Cλ and Cκ, respectively.
Fig 3.
Phylogenetic tree analysis of the 86 Alligator sinensis Vλ genes.
A phylogenetic tree of the nucleotides of 86 Alligator sinensis Vλ segments was constructed. The 19 Vλ gene families are labeled with numbers on the right. Phylogenetic trees were constructed using MrBayes3.1.2 [58] and viewed in TREEVIEW [59].
Fig 4.
Phylogenetic tree analysis of the 62 Alligator sinensis Vκ genes.
A phylogenetic tree of the nucleotides of the Alligator sinensis Vκ segments was constructed. The 12 Vκ gene families are labeled with numbers on the right. Phylogenetic trees were constructed using MrBayes3.1.2 [58] and viewed in TREEVIEW [59].
Fig 5.
Phylogenetic analysis of the IgL chain C genes in jawed vertebrates.
The phylogenetic tree was constructed using C domains. The scale shown as a bar represents the genetic distance (number of nucleotide changes at the given scale). The credibility value for each node is shown. Phylogenetic trees were constructed using MrBayes3.1.2 [58] and viewed in TREEVIEW [59].
Fig 6.
Phylogenetic analysis of the IgL chain V genes in jawed vertebrates.
The phylogenetic tree was constructed using V domains. The scale shown as a bar represents the genetic distance (number of nucleotide changes at the given scale). The credibility value for each node is shown. Phylogenetic trees were constructed using MrBayes3.1.2 [58] and viewed in TREEVIEW [59].
Fig 7.
Chromosomal locations of the λ genes in different species and type III genes in X. tropicalis.
Arrows indicate the transcriptional orientation of the genes. Chr: chromosome; IGLC: immunoglobulin λ chain constant region gene; GNAZ: guanine nucleotide-binding protein, α z subunit; HIRA: histone cell cycle regulation defective homologue A; MRPL40: mitochondrial ribosomal protein L40; RTDR1: rhabdoid tumor deletion region gene 1. The figure was modified from Ref. [31].
Fig 8.
Chromosomal locations of the κ genes in different species and ρ genes in X. tropicalis.
Arrows indicate the transcriptional orientation of the genes. Chr: chromosome; IGκC: immunoglobulin κ chain constant region gene; RPIA: ribose-5-phosphate isomerase A; EIF2AK3: eukaryotic translation initiation factor 2-α kinase 3; SUCLG1: succinate-CoA ligase, GDP-forming, α subunit; SLC4A11: solute carrier family 4, sodium borate transporter, member 11; SLC9A4; solute carrier family 9, sodium borate transporter, member 4; RPL31: ribosomal protein L31; RPL19-PS: ribosomal protein L19, pseudogene 8; RPL19: ribosomal protein L19. SLC4A1: solute carrier family 4, sodium borate transporter, member 1. The figure was modified from Ref. [31].
Fig 9.
Usage frequency of Vλ and Jλ genes in the Alligator sinensis.
The number behind the Vλ indicates the number of the family.
Fig 10.
Usage frequency of Vκ and Jκ genes in the Alligator sinensis.
The number behind the Vκ indicates the number of families.