Table 1.
Multiple parameters of serum samples and statistical analyses between groups.
Fig 1.
Negative correlation between the concentrations of UA and TRACP 5b.
(A) The average TRACP 5b levels of the 77 high-UA individuals (1.47± 0.62 U/L) were significantly lower (t-test, p<0.0001) than that of the 77 healthy subjects (2.62 ± 0.63U/L). (B) The average TRACP 5b levels of the 16 female high-UA individuals (1.51 ± 0.64 U/L) were significantly lower (t-test, p<0.0001) than that of the 16 female healthy subjects (2.73 ± 0.86 U/L). (C) The average TRACP 5b levels of the 61 male high-UA individuals (1.46 ± 0.62 U/L) were significantly lower (t-test, p<0.0001) than that of the 61 male healthy subjects (2.59 ± 0.56 U/L). Triple asterisk indicates p<0.0001.
Fig 2.
High concentrations of UA lead to decrease of TRACP 5b levels.
A TRACP 5b standard sample was divided into 6 aliquots. Serial dilutions of UA (250, 500, 1000, 2000, 4000μM) were prepared from UA standard subject. The 5 aliquots were then spiked at 3:2 with each UA solution. This generated 5 different test samples with the same TRACP 5b level, whose final UA concentrations ranged from 100 to 1600 μM. An aliquot containing DDW instead of UA served as a blank. The average TRACP 5b concentration is marked with error bars representing standard deviations of three independent experiments.
Fig 3.
Derivation of a UA correction equation.
The TRACP 5b concentrations of the six distinct test samples with the same TRACP 5b standard sample and final UA concentrations ranging from 0 to 1600 μM were measured. ΔTRACP 5b means difference of TRACP 5b concentrations measured between the samples of final UA concentrations ranging from 100 to 1600 μM and that of no UA. The average ΔTRACP 5b concentration is marked in figure with error bars representing standard deviations.
Fig 4.
Correction of decreased TRACP 5b concentrations resulting from high-UA individuals compared to healthy subjects.
Each high-UA individual's UA-corrected TRACP 5b concentration was calculated based on the UA-TRACP 5b correction equation. Increasing UA concentration means difference value between each high-UA individual's and mean value of healthy subjects’. Triple asterisk indicates p<0.0001. “N.S” indicates no significant difference.