Fig 1.
Two Canna cultivars having contrasting flower color (A) Tropical sunrise (B) Red president.
Fig 2.
Flavonoids, Carotenoids, Xanthophyll and Anthocyanin content of TS and RP.
All the measurements were taken in three biological and five technical replicates. Error bars represent standard deviation of biological and technical replicates.
Fig 3.
Length distribution of sRNAs in two libraries, TS and RP.
Two sRNA libraries were prepared from staminode tissue of two contrasting flower cultivars of Canna. Size distribution of the sRNA libraries show that the 21 nt size miRNAs were dominating ones in both the libraries.
Table 1.
Categorization and abundance of sRNA reads from TS and RP.
Fig 4.
Venn diagrams of conserved and unique miRNAs between TS and RP.
(A) Total conserved and unique miRNAs between TS and RP, (B) Conserved and unique miRNA families between TS and RP, (C) Total conserved and unique miRNA* between TS and RP, (D) Conserved and unique miRNA* families between TS and RP.
Fig 5.
Number of miRNAs members in each family identified from TS and RP by deep sequencing.
Fig 6.
The homology of identified miRNAs with other plant species.
Values on Y axis indicate the number of homological miRNA families between Canna and other plant species.
Fig 7.
Differentially expressed miRNA families between TS and RP.
Fig 8.
Comparison of the miRNA expression levels determined by deep sequencing and qRT-PCR.
Blue and red colors indicate the fold change obtained by deep sequencing and qRT-PCR, respectively. The error bars indicate the standard deviation obtained from biological and technical replicates.
Fig 9.
Detection of cleavage site through RLM-RACE.
5' RLM RACE was used to map the cleavage sites. The partial mRNA sequence from the target genes were aligned with the miRNA. The arrow indicates the cleavage site, and the number above the arrow denotes the frequency of the sequenced clones.
Fig 10.
Correlation of miRNAs and their target genes expression in TS and RP.
The target genes of selected miRNAs were analyzed by qRT-PCR. The expression level of these target genes (red) were compared with the expression level of corresponding miRNAs (blue) estimated from sequencing data. Error bars indicate mean ± standard deviation of three biological and three technical replicates.
Fig 11.
Gene ontology enrichment analysis of the predicted targets of Canna miRNAs.
Categorization of miRNA-targets genes were performed according to the(A) Biological Processes, (B) Molecular function and (C) Cellular component.