Fig 1.
Overview of the Celsee Diagnostics CTC system.
(A) Celsee PREP 400 system (left) for blood sample processing and Celsee Analyzer (right) for image analysis. (B) Design of the Celsee PREP 400 microfluidic flow. (C) Design of microfluidic chip (top) and mechanism of capturing CTCs with inlet and outlet for pumping blood samples and reagents through the device (bottom). CTCs are captured in microchannel chambers of the chip while red blood cells and most leukocytes go through the microchannel chambers into the outlet to achieve separation.
Fig 2.
Detection and enumeration of CTCs in blood samples from patients with metastatic breast, prostate and colorectal cancer.
Number of CTCs (Y-axis) in 2ml of blood captured by the Celsee PREP 400 system from healthy donors and patients with metastatic breast, prostate and colorectal cancer.
Fig 3.
Comparison of CTC counts using the Celsee PREP 400 microfluidic device and the CellSearch system.
Number of CTCs measured by either Celsee PREP 400 or CellSearch are standarized to CTCs per 7.5 ml of blood. Twenty samples from patients with metastatic prostate cancer are included in the study.
Fig 4.
Immunostaining of enriched CTCs from cancer patients.
(A). A representative image of staining by DAPI, panCK and CD45 antibodies. CTCs are identified by the following criteria: DAPI+ (blue), panCK+ (green) and CD45- (red). (B) Immunofluorescence staining of enriched cells with anti-vimentin. A representative image of staining for DAPI (blue) and vimentin (green). (C) Immunofluorescence staining of enriched cells with anti-PSA. A representative image of staining for DAPI (blue) and PSA (green).
Fig 5.
DNA FISH and mRNA FISH analysis of enriched CTCs from metastatic breast cancer patients.
(A) DNA FISH of enriched CTCs. A representative image of CTC hybridized to Her-2 (green), Chromosome 17 (red) probes and stained by DAPI (blue). (B) mRNA FISH of enriched CTCs. A representative image of CTC (left panel) and a leukocyte (right panel) hybridized to panCK (green), CD45 (red) probes and stained by DAPI (blue).