Table 1.
Characteristics of subjects.
Fig 1.
Changes in systolic (A) and diastolic (B) blood pressure levels after drinking alcohol.
Blood pressure was measured just before dinking and at 45 min and 2–3 hr after drinking. Means ± standard errors of blood pressure levels are shown. Asterisks denote significant differences from the levels before drinking (**, p < 0.01).
Fig 2.
Differential profiling of serum sampled from subjects before and after drinking.
Each integrated spectrum normalized with ClinPro Tools version 2.2 is the average of 10 samples (total of 44 integrations) from subjects.
Fig 3.
MS/MS structural analysis of m/z 1467 (A), 2380 (B), 2662 (C) peptides in the sample after reversed-phase high-pressure liquid chromatography separation.
Table 2.
Profiles of the identified peptide fragments showing quantative changes in relation to changes in blood pressure after alcohol drinking.
Fig 4.
Changes in intensities of m/z 1467 (A), 2380 (B) and 2662 (C) peptides after drinking alcohol.
Medians with 25 and 75 percentile values of intensities of the peptides are shown using box plots. Intensities of each peptide in serum collected just before dinking and at 45 min and 2–3 hr after drinking were measured by mass spectrometry using BLOTCHIP®. Symbols denote significant differences from the levels before drinking (*, p < 0.05; **, p < 0.01) and the levels at 45 min after drinking (†, p < 0.05; ††, p < 0.01). #, a marginally significant difference from the intensity before drinking (p = 0.074).
Fig 5.
Scatter plots of the relationships between ranks of serum m/z 2380 peptide intensity before drinking and % changes in systolic (A) and diastolic (B) blood pressure at 45 min after drinking.
Spearman’s rank correlation coefficients (r) are given in the figures.
Table 3.
Correlations between each peptide level in blood before drinking and changes in systolic and diastolic blood pressure levels before and after drinking.
Fig 6.
A. Changes in blood complement C4a level after drinking alcohol. Complement C4a concentration was measured just before drinking and at 45 min and 2–3 hr after drinking. Means ± standard errors of complement C4a levels are shown. An asterisk denotes a significant difference from the level before drinking (*, p < 0.05). B,C. Scatter plots of the relationships between blood complement C4a level before drinking and % changes in systolic (B) and diastolic (C) blood pressure at 45 min after drinking.
Pearson’s correlation coefficients (r) are given in the figures.