Table 1.
Sequence of primers for PCR amplification used throughout these studies.
Table 2.
E. coli strains used in these studies.
Fig 1.
P. caudatum grazing of E. coli O157:H7 EDL933 and cattle commensal E. coli (186) over three days at ambient laboratory temperature.
CFU (mean ± s. e.) for three grazing trials each containing a matched MOI of E. coli O157:H7 EDL933 (squares) and cattle commensal E. coli (triangles).
Fig 2.
Ratios of CFU of five strains of E. coli O157:H7 compared to matched MOI of cattle commensal E. coli (186) grazed by P. caudatum over three days at ambient laboratory temperature.
Five strains of E. coli O157:H7 (Table 2) containing different Shiga toxin profiles: EDL933 (squares), E12056 (diamonds), E12058 (circles), E12061 (triangles), E12064 (X).
Fig 3.
Density (mean ± s. e.) of P. caudatum when co-cultured with matched MOI of E. coli O157:H7 EDL933 and cattle commensal E. coli (186) for three days at ambient laboratory temperature.
Squares, P. caudatum with added E. coli; no marker, P. caudatum in trough water without added bacteria.
Fig 4.
CFU (mean ± s. e.) of E. coli O157:H7 EDL933 and isogenic mutants inoculated at matched MOI when co-cultured with T. pyriformis (solid lines) and P. caudatum (dashed lines) at ambient laboratory temperature.
(A) E. coli O157:H7 EDL933 (squares) and EDL933ΔStx 2a subunit A (triangles). (B) E. coli O157:H7 EDL933 (squares) and EDL933ΔStx 1 phage (diamonds). (C) E. coli O157:H7 EDL933 (squares) and EDL933ΔStx 2a phage (circles). (D) E. coli O157:H7 EDL933 (squares) and EDL933ΔStx 1 & Stx 2a phages (asterisks).