Fig 1.
Relationships of the z-score with cff DNA fractions in artificial DNA mixture samples.
The z scores of chromosome 13 (A), 18 (B) and 21 (C) are compared among three kinds of cff DNA fractions about 3.5%, 5% and 10%, respectively.
Fig 2.
Relationships between cff DNA fractions and parameters of pregnancies.
Solid lines in red show the trends between cff DNA and maternal age (A) as well as gestational age (B).
Fig 3.
Relationships of the z-score with fetal DNA fractions in maternal plasma samples.
Dash line marks the z-score cutoff of 3 for detecting trisomies of chromosome 21(A), 18(B), 13(C). Solid line markers shows the correlations between the z score and cff DNA fractions for the trisomy 21, 18, 13 cases (blue) and negative cases of chromosome 21, 18 and 13(green). Depictions are positive cases of pregnancies bearing fetuses with trisomy 21, 18, 13 (red triangles) and their negative cases (green circles).
Fig 4.
Size distributions of sequencing libraries and cff DNA fractions between group A and group B.
The size distributions of DNA sequencing libraries are showed for group A (A), in which the large-size DNA fragment was discarded during library construction, and group B (B), in which it was retained. The cff DNA fractions are compared between the paired clinical samples in group A and group B (C).