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Fig 1.

Color variation in two samples stained with Masson’s trichrome.

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Fig 2.

Flow chart of image pre-processing stage.

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Fig 3.

CIELab image.

(A) Vessel wall image; (B) Histogram of the lightness component.

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Fig 4.

Pre-processing stage.

(A) Tissue folds detected; (B) Blood residues detected; (C) Lightness component of the contrast-enhanced image after removing defects; (D) Binary image thresholded by Otsu’s method; (E) DBSCAN algorithm output; (F) Vessel wall area image.

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Fig 4 Expand

Fig 5.

Flow chart of image feature extraction stage.

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Fig 6.

Process of the k-means algorithm.

(A) Input image; (B) SMF component detected; (C) ECM component detected; (D) SMF area; (E) ECM area.

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Fig 7.

Characteristics of the database.

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Fig 8.

Normalized measures of different vein segment types.

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Table 1.

Execution time of permutation test (n1 = 22 and n2 = 20).

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Table 2.

p-values from experiments.

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Table 2 Expand

Fig 9.

State-of-the-art in varicose vein studies.

1 h: healthy sample, c: competent sample, i: incompetent sample 2 vv: varicose samples (competent and incompetent). <s or >s: the difference indicated is statistically significant (α = 0.05). < or >: the difference indicated is not statistically significant.

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