Fig 1.
Identification of translocation chromosomes.
(A)–(D), FISH and GISH using rye genomic DNA (red), pSc119.2(green), and pAs1 (red) as probes. (A) and (B), Wheat and rye parents. (C) and (D), new primary 1RS.1BL translocation lines. (E), FISH using 6c6 (green) and telomere sequence (red) as probes, indicating the integrity of all chromosomes. (F), FISH using 6c6 (green) and pAWRC.1 (red) as probes, indicating that the 1RS arm is intact in the translocation.
Fig 2.
A-PAGE separations of ω-secalins and gliadins from a new primary 1RS.1BL translocation line and its wheat and rye parents.
(L to R) 1 = wheat parent A42912, 2 = Weining rye, 3 = Chuan-nong 10, 4 = Chuan-nong 17, 5–8 = different lines of T917-26, the seeds from two years, 9 and 10 = different lines of T917-15. Arrowheads indicate the absence of expression of ω-secalins.
Table 1.
Effects of expression deletion mutation of Sec-1 genes in primary 1RS.1BL lines on the quality traits.