Fig 1.
Treatment schedule of the experimental study.
Table 1.
The chronic mild stress protocol during the first week of the experiment.
This schedule were followed for another three week.
Fig 2.
Effect of stress on the levels of dopamine in control and MPTP/p treated mice.
The levels of dopamine in striatum, cortex and hippocampus were determined by HPLC- electrochemical method. Values are given as mean ± SD for six mice in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 3.
Impact of stress on the levels of serotonin in control and MPTP/p treated mice.
The levels of serotonin in nigrostriatal (A) and extra-nigrostriatal (B) were determined by HPLC- electrochemical method. Values are given as mean ± SD for six mice in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 4.
Effect of stress on immunoreactivity of TH positive cells in control and MPTP/p treated mice.
Immunoreactivity of TH positive cells (A) in SN and quantified data (B) were shown. Values are given as mean ± SD for three mice in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 5.
Effect of stress on protein expression of TH in striatum and SN of control and -MPTP/p treated mice.
Protein expression of TH (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 6.
Impact of stress on protein expression of DAT in striatum and SN of control and -MPTP/p treated mice.
Protein expression of DAT (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 7.
Influence of stress on protein expression of VMAT 2 in striatum and SN of control and -MPTP/p treated mice.
Protein expression of VMAT 2 (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 8.
Impact of stress on protein expression of TH in cortex, hippocampus and cerebellum of control and -MPTP/p treated mice.
Protein expression of TH (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 9.
Influence of stress on protein expression of DAT in cortex, hippocampus and cerebellum of control and MPTP/p treated mice.
Protein expression of DAT (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 10.
Effect of stress on protein expression of VMAT 2 in cortex, hippocampus and cerebellum of control and MPTP/p treated mice.
Protein expression of VMAT 2 (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 11.
Effect of stress on protein expression of α-synuclein in striatum and SN of control and MPTP/p treated mice.
Protein expression of α-synuclein (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 12.
Effect of stress on protein expression of α-synuclein in cortex, hippocampus and cerebellum of control and MPTP/p treated mice.
Protein expression of α-synuclein (A) and quantified data (B) by densitometric analysis were shown. Values are expressed as arbitrary units and given as mean ± SD of three animals in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 13.
Effect of stress on open field behavior of control and MPTP/p treated mice.
The locomotion (peripheral (A) and central (B) movements) and activities (C) (grooming and rearing) were shown. Values are given as mean ± SD for six mice in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 14.
Effect of stress on narrow beam walking test of control and MPTP/p treated mice.
Time taken to cross the wooden beam (A) and number of foot slip errors (B) in narrow beam walking test were shown. Values are given as mean ± SD for six mice in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.
Fig 15.
Effect of stress on sucrose intake levels in control and MPTP/p treated mice.
The level of sucrose intake was shown. Values are given as mean ± SD for six mice in each group. Values not sharing common alphabet differed significantly (P< 0.05) with each other.