Table 1.
List of samples.
Table 2.
List of primers.
Table 3.
PCR results showing Staphylococcus aureus strains.
Table 4.
PCR results showing Staphylococcus epidermidis strains.
Fig 1.
Detection of biofilm formation in Staphylococcus aureus strains.
All strains were incubated for 1 hour and 24 hours in TSB-gluc and absorbance was detected at 595 nm using a microplate reader. All data are shown as geometric means from three independent experiments with standard deviations.
Fig 2.
Detection of biofilm formation in Staphylococcus epidermidis strains.
All strains were incubated for 1 hour and 24 hours in TSB-gluc, and absorbance was detected at 595 nm using a microplate reader. All data are shown as geometric means from three independent experiments with standard deviations.
Fig 3.
Biofilm formation in glass vials.
(A) Biofilm formation from Staphylococcus aureus strains in glass vials with TSB-gluc after overnight incubation. (B) Biofilm formation from Staphylococcus epidermidis strains in glass vials with TSB-gluc after overnight incubation.
Fig 4.
Ring test to evaluate biofilm formation.
(A) Biofilm formation in Staphylococcus aureus based on ring test in glass vials using crystal violet solution; sample number 82 is the ATCC control sample. (B) Biofilm formation in Staphylococcus epidermidis based on ring test in glass vials using crystal violet solution; sample number 83 is the ATCC control sample.
Fig 5.
Binding of Staphylococcus aureus to live human colonic epithelial cells at 4°C to prevent bacterial invasion.
All data are shown as the average of cell-associated bacteria ± standard error from four independent experiments. Statistical significance of the differences is indicated in brackets.
Fig 6.
Binding of Staphylococcus epidermidis to live human colonic epithelial cells at 4°C to prevent bacterial invasion.
All data are shown the as average of cell-associated bacteria ± standard error from four independent experiments. Statistical significance of the differences is indicated in brackets.
Fig 7.
Visualization of bacterial attachment to formalin fixed Caco-2 cells using Giemsa staining.
Fig 8.
Breast prostheses analyzed by scanning electron microscope.
Letters (A) magnification 2000X, (B), (C), (D) represent different images of breast prostheses in which biofilm formation can be observed with 4000X of magnification. (E) Negative control breast prostheses without any traces of biofilm formation.