Fig 1.
The Respiratory Aerosol Sampling Chamber (RASC).
(A) Photograph of the RASC (with the door open) on site in a community TB clinic (1) aerodynamic particle sizer (2) Filter samplers (3) Andersen impactor (4) Mixing fan (5) CO2, temperature and RH (6) PM10 impactor (7) Chair for participant. (B) Block diagram depicting the fluidic and electronic configuration of the RASC. Thick connecting lines indicate airflow and aerosol paths; thin lines indicate electronic connections. All air leaving the RASC is HEPA filtered.
Fig 2.
The in-built APS characterizes the particle size distribution spectrum within the RASC.
(A) Typical background particle spectrum before and after air wash. Note the 10-fold decrease in particle counts across all size ranges following the air wash. Total count from a typical five seconds of sampling. (B) Artificial dry release of fluorescent polystyrene latex (PSL) microspheres. The APS instrument groups all particles with an aerodynamic diameter less than 0.523 μm in the number bin on the far left of each chart. Note that the 1μm release (b) contained approximately ten times more particles in the release. Total count from ten seconds of sampling at the peak of particle concentration. (Inset) corresponding SEM images of the released particles.
Fig 3.
The effect of aerosol hydration on particle size distribution.
The graph shows APS measurements of “wet” and “dry” releases of M. smegmatis::gfp. (see text for details).
Fig 4.
Isolation and visualization of viable mycobacteria in the RASC.
(A) M. smegmatis::gfp growth on solid 7H10 agar plates from the Six-Stage Viable Andersen Cascade Impactor after wet release of 200 μl diluted culture into the RASC (30 000, 3000 and 300 colony forming units—CFU). The columns indicate the particle sizes captured on each plate across the 6 stages of the impactor, and the rows indicate the estimated total number of CFU passing through the impactor. Each release was repeated three times and the mean and SD for each plate are presented below the typical growth pattern distribution seen in the particle release. In all the releases the sampling was run for 5 minutes at 28 l/min resulting in the potential total capture of 3000, 300 and 30 CFU respectively. (B) SEM (left) and fluorescent microscopy (right) of M. smegmatis::gfp isolated on a PM10 impactor following experimental release.
Table 1.
Sampling rates of the capture devices and the periods in which they are used.
Fig 5.
Particle production as a function of respiration in a clinical TB patient.
CO2 concentration (solid line and left ordinate) and particle counts (dots and right ordinate) in the 1–2.5 μm size range for a TB patient.
Fig 6.
Isolation of Mtb from a TB patient.
SEM image of patient sample impacted on the lower plate of the PM10 impactor. The dimensions and morphology of the rod-shaped structure (denoted by *) are consistent with the presence of Mtb bacilli in the untreated TB patient. There is also evidence of multiple “splats” of unknown identity (one example is denoted by **) which might comprise organic matter derived from patient lung or respiratory tract. Note the “halo” structures (dark shadows) surrounding each particle.