Fig 1.
In situ transmission experiment.
Replicate fragments of resistant and susceptible corals were exposed to allogeneic tissue and to active WBD using healthy grafts (a) and WBD grafts (b) and placed in common gardens (c).
Fig 2.
Differential expression analyses of RNA-seq data for staghorn coral resistance phenotypes and exposure treatments.
Number of coral transcripts exhibiting significant differential expression (adj p-val<0.05) due to the resistance phenotype (resistant vs susceptible) and to exposure (allogeneic graft vs control; disease graft vs control). Only five transcripts were significant in both treatments (R+E, see also S1 Table). No significant interaction was found between resistance and exposure (R*E = 0).
Fig 3.
Gene expression analysis of resistant (R) and susceptible (S) corals exposed to allogeneic tissue and active White Band Disease (WBD).
Clustered heatmap for gene expression of the 35 annotated differentially expressed genes that differ between resistant (R) and susceptible (S) corals exposed to WBD grafts (D), healthy grafts (H) and unexposed (no graft) controls (C). Hierarchical clustering was performed using Pearson correlation metrics and indicates similarity between the different samples (columns), with resistant and susceptible samples forming different clusters. Red corresponds to up-regulated genes, blue indicates down-regulation. The expression level for each transcript was calculated as log2 fold change of normalized count data. Asterisks indicate transcripts that were DE in both factors (resistance + exposure). See also S1 Table.