Table 1.
Clinical and pathological features of 163 invasive early-stage breast carcinoma.
Fig 1.
A: Estrogen-Receptor status (ER). Comparison between the Immunohistochemistry analysis (IHC) and the MapQuant test. Top. ER status determined by IHC referred to MapQuant test. Green: IHC-negative tumours. Red: IHC-positive tumours. Vertical axis: MapQuant test values. 4 IHC-negative cases were positive with the MapQuant test. Bottom. MapQuant determination of the ER status. Green: negative. Black: equivocal. Red: positive. Both equivocal cases corresponded to IHC-positive tumours. B: Progesterone-Receptor status (PR): comparison between the Immunohistochemistry analysis (IHC) and the MapQuant test. Top. PR status determined by IHC referred to MapQuant test. Green: IHC-negative tumours. Red: IHC-positive tumours. Vertical axis: MapQuant test values. 11 IHC-negative cases were positive with the MapQuant test. Bottom. Determination of the PR status by the MapQuant test. Green: negative. Black: equivocal. Red: positive. Equivocal cases corresponded to 15 IHC-positive tumours and 20 IHC-negative tumours. C: HER2 status.
Correlation between the Immunohistochemistry analysis (IHC) and the MapQuant test. Top. HER2 status determined by IHC referred to MapQuant test. Green: IHC-negative tumours. Red: IHC-positive tumours. Vertical axis: MapQuant test values. Only one IHC-negative case was found positive with the MapQuant test. Bottom. Determination of the HER2 status by the MapQuant test. Green: negative. Black: equivocal. Red: positive. Equivocal cases corresponded to 2 IHC-negative tumours.
Fig 2.
ER (A) and PR (B) expression by IHC and MapQuant test.
Y axis: MapQuant values. X axis: Immunohistochemistry measure of ER and PR as percentage of stained tumour cells. Threshold for IHC positive sample is indicated by the vertical line (10%). MapQuant status. Green dot: negative. Black dot: equivocal. Red dot: positive.
Fig 3.
IHC pictures of 4 discordant cases for ER status.
Patients (A-D) were IHC-negative/MapQuant-positive. Patient (E) was IHC-negative/MapQuant-negative. Patient (F) was IHC-positive 100%/MapQuant-positive.
Fig 4.
IHC picture of one discordant case for HER2 status.
Patient (A) was IHC-negative/MapQuant-negative. Patient (B) was IHC-negative/MapQuant-positive. Patient (C) was IHC-positive/MapQuant-positive.
Table 2.
MapQuant test values of Estrogen-Receptor (ER), Progesterone-Receptor (PR) and HER2 status compared to immunohistochemistry (IHC).
Table 3.
Concordance (%) of the main Gene Expression Assays in determining ER, PR and HER2 status compare to corresponding IHC reference.