Fig 1.
Technical drawing of the setup.
It consists of a (10 × 8 × 1.88)mm3 micro-NMR probe, and of a (2 × 16 × 0.6) mm3 exchangeable microfluidic application-specific sample insert (ASSAI) with a total sample volume of 2 μL, with the analyte indicated in red.
Fig 2.
Schematic illustration of the ASSAI fabrication process.
(a) Multiple lamination of DFR on bottom wafer. (b) UV exposure of dry film resist and development. (c) Laser-drilling of top wafer. (d) Full-wafer bonding.
Fig 3.
Photograph of bonded wafers before dicing.
The stack contains 129 ASSAIs.
Fig 4.
Schematic illustration of the probe fabrication process.
(a) Electroplating of Au. (b) Patterning of dry film resist. (c) Wirebonding of micro-coils. (d) Encapsulation of coils and glueing of glass spacers. (e) Flip-chip bonding of top and bottom chips. (f) Soldering of electrical interconnects.
Table 1.
Employed values of the components illustrated in S1 Fig.
Capacitances Ci are given in pF, while inductances Lj are given in nH.
Fig 5.
Helmholtz microcoil integrated onto a PCB.
The setup was mounted on a custom made sample holder, designed to fit onto a commercial Bruker Micro 5 probe base.
Fig 6.
SNR map and extracted profiles along two perpendicular axes.
(a) SNR map, coronal xz-plane. (b) Extracted SNR values along the x-axis and simulated B1 profile. (c) Extracted SNR values along the z-axis and simulated B1-field profile.
Fig 7.
1D proton spectrum of 100mM sucrose in D2O.
The inset shows the doublet of the anomeric proton.
Fig 8.
(a) Pure ethanol. (b) 1 M sucrose in D2O.
Fig 9.
CSI-ASSAI, extracted spectra and assigned peaks.
(a) CSI-ASSAI filled with aqueous ink (left) and overlay of morphological reference and CSI spectra (right). (b) Extracted and assigned spectra from the two voxels marked in the right half of (a).