Table 1.
Clinicopathological characteristics in colorectal and prostate cancer subjects analyzed in this study.
Fig 1.
Electrophoregrams of co-amplified homologous sequences.
Electrophoregrams from subjects with a) normal amelogenin Y/X ratio (approximately equal peak areas of X chromosome and Y chromosome sequences) and b) patient with colorectal cancer and significantly increased proportion of cells with LOY (amelogenin Y/X ratio of 0.28).
Fig 2.
Scatter plot of amelogenin Y/X ratio obtained from different proportions of 45,X0 and 46,XY DNA samples.
Dots represent averaged amelogenin Y/X ratio values corresponding to 0%, 2.5%, 5%, 10%, 15%, 20%, 30%, 50%, 70% and 90% of the 45,X0 in the mixed DNA samples. Regression line was estimated using percentage of 45,X0 sample as independent and amelogenin Y/X ratio as dependent variable. Slope (s) and standard deviation (σ) of the regression line were used to calculate the limit of detection using the formula LOD = 3.3σ/s [28].
Fig 3.
Histograms of distribution of Y/X ratios in colorectal and prostate cancer patients and controls.
Mean values and standard deviations are also shown.
Table 2.
Multivariable logistic regression analysis for cancer presence adjusted for age and LOY (measured with amelogenin Y/X ratio).