Fig 1.
A map of a study area near Busselton, Western Australia.
Red lines represent the borders of Caves Road (west to east) and blue lines represent the borders of an artificial waterway (north to south). 1A, 1B, 1-2C, 1D, 2A, 2B and 2D are 200 m × 200 m study blocks where samples from Pseudocheirus occidentalis were collected. 1A, 1-2C and 2A were inside Locke Nature Reserve, and 1B, 1D, 2B and 2D were within partially cleared campsites. The aerial photograph of the study area is used with permission from Western Australian Land Information Authority (Midland, WA Australia).
Table 1.
Genetic variation among Pseudocheirus occidentalis within each study block.
Fig 2.
(a) A correlogram plot and (b) a multiple distance class plot based on 69 Pseudocheirus occidentalis in continuous habitat in Locke Nature Reserve near Busselton, Western Australia.
Dotted lines (a) and small blue markers (b) represent upper and lower 95% confidence intervals around zero. Red circle markers on the solid line (a) and red markers (b) are the genetic correlation values (r) that differ significantly from zero based on bootstrap resampling. Sample sizes are shown in parentheses.
Fig 3.
Plot of two-dimensional local spatial autocorrelation analyses of Pseudocheirus occidentalis sampled in Locke Nature Reserve near Busselton, Western Australia.
Red lines represent the borders of Caves Road (west to east) and blue lines represent the borders of an artificial waterway (north to south). The area south of Caves Road and west of the waterway is Locke Nature Reserve. Markers represent geographical locations of the local spatial autocorrelation analyses with significantly positive (solid symbols) or non-significant values (open symbols) based on five nearest neighbours. Coordinates are based on GDA 94 projection (zone 50).
Table 2.
Pairwise FST values (below diagonal) and P-values from tests of differentiation (above diagonal) between blocks.
Fig 4.
Summary of ΔK estimates[30] for varying numbers of genetic clusters (K) derived from the STRUCTURE analysis of 145 adult Pseudocheirus occidentalis from Busselton, Western Australia.
Fig 5.
Summary of the Bayesian clustering analysis assuming three admixed populations of Pseudocheirus occidentalis in Busselton, Western Australia.
Each column represents an individual’s estimated membership to three genetic clusters represented by different colours. Vertical dotted lines separate individuals sampled from different blocks. Vertical bold solid lines represent the presence of Caves Road and an artificial waterway.
Fig 6.
Mean pairwise relatedness values [34] for Pseudocheirus occidentalis individuals sampled from the same block (“Within”), different blocks on the same side of an artificial barrier (“Same”) and opposite sides of an artificial barrier (“Opposite”).
Error bars represent the 95% confidence levels determined by bootstrap resampling, and values in parentheses are the numbers of pairwise comparisons from which the average pairwise relatedness values were calculated. Asterisks represent mean values significantly different to zero determined by a permutation test.