Table 1.
Effect of SB-710411 on hemodynamic parameters of rats subjected to the left anterior descending artery occlusion and reperfusion.
Fig 1.
Effects of SB-710411 on ischemia/reperfusion injury-induced myocardial infarction in rats.
Evans Blue and 2,3,5-triphenyltetrazolium chloride (TTC) double staining was used to determine the ischemic size and infarction size. A. Rat myocardial slice stained by Evans blue and TTC. The normal area was stained blue, the area at risk (AAR) was stained red, and the area of infract size (IS) was stained pale white. a: Sham; b: Control; c: 1.6 mg/kg verapamil; d ~ f: 0.5, 1.0 and 2.0 μg/kg SB-710411. B. Effect of SB-710411 on the myocardial infarction. The IS was normalized by expressing it as a percentage of the AAR (IS/AAR). C. Effect of SB-710411 on the AAR. AAR was expressed as a percentage of the left ventricular area (AAR/LV). Results are expressed as mean ± SD (n = 8 per group). **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.01 vs. the control group.
Fig 2.
Effect of SB-710411 on ST-segment elevation after myocardial ischemia/reperfusion in rats.
Rats were subjected to 30 min of myocardial ischemia followed 90 min of reperfusion, and electrocardiogram (ECG) was measured. A. The traces of the ECG in various groups. a: Sham; b: Control; c: 1.6 mg/kg verapamil; d ~ f: 0.5, 1.0 and 2.0 μg/kg SB-710411. B. Effect of SB-710411 on ST-segment elevation. Results are expressed as mean ± SD (n = 8 per group). **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.01 vs. the control group.
Fig 3.
Effect of SB-710411 on the serum creatine phosphokinase-MB (CK-MB) and lactate dehydrogenase (LDH) activities and cardiac troponin I (cTnI) level in rats subjected to 30 min of ischemia followed 90 min of reperfusion.
A. CK-MB activity. B. LDH activity. C. cTnI level. Results are expressed as mean ± SD (n = 8 per group). **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.01 vs. the control group.
Fig 4.
Ischemia/reperfusion-induced the histopathological alteration in rat myocardium (hematoxylin and eosin staining, magnification 200×).
The black bar represents 50 μm. Histological analysis showed that there is normal intact architecture of the myocardium in the sham group, while an irregular arrangement, obvious necrosis, marked edema, neutrophilic infiltration, mild inflammation, myofibril loss and cracks were observed in the control group. 1.0 and 2.0 μg/kg SB-710411 and verapamil 1.6 mg/kg significantly alleviated the myocardial damage. a: Sham; b: Control; c: 1.6 mg/kg verapamil; d ~ f: 0.5, 1.0 and 2.0 μg/kg B-710411.
Table 2.
Effect of SB-710411 on myocardial histologic scores of rat subjected to the left anterior descending artery occlusion and reperfusion.
Fig 5.
Effect of SB-710411 on the expression of UTR protein in rat myocardium after ischemia/reperfusion.
A. UTR protein was detected by Western blot method. β-actin was used as the loading control. B. Densitometric quantification of UTR protein expression. a: Sham; b: Control; c ~ e: 0.5, 1.0 and 2.0 μg/kg SB-710411. Results are expressed as mean ± SD (n = 4 per group). **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.01 vs. the control group.
Fig 6.
Effect of SB-710411 on RhoA activity in rat myocardium after ischemia/reperfusion.
The activity of RhoA in myocardium was detected by luminescence-based G-LISA™ assay. Results are expressed as mean ± SD (n = 4 per group). **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.01 vs. the control group.
Fig 7.
Effect of SB-710411 on protein expressions of ROCK1 and ROCK2 in rat myocardium injured by ischemia/reperfusion.
A. ROCK1 and ROCK2 proteins were detected by Western blot method. β-actin was used as the loading control. B. Densitometric quantification of ROCK1and ROCK2 protein expression. a: Sham; b: Control; c ~ e: 0.5, 1.0 and 2.0 μg/kg SB-710411. Results are expressed as mean ± SD (n = 4 per group). **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.01 vs. the control group.