Table 1.
GISH detection of the M1BC1 and F2 plants.
Table 2.
The translocation types and frequencies of wheat-A. cristatum chromosome 2P translocation.
Fig 1.
GISH patterns of some plants with wheat-A. cristatum 2P translocated chromosomes.
A. cristatum genomic DNA was labelled with digoxigenin-11-dUTP red fluorescence and the wheat DNA was counterstained with DAPI blue fluorescence. Arrows show the translocated chromosomes. A: Small alien segmental translocation and a 2P chromosome. B: Large alien segmental and whole-arm translocation. C: Large and small alien segmental translocation. D: Large alien segmental translocation. E: Two small alien segmental and intercalary translocation. F: Whole-arm reciprocal translocation. G: Whole-arm and small alien segmental translocation. H: Whole arm and intercalary translocation. I: Whole-arm translocation. J1: Whole-arm translocation. J2: Large and small alien segmental translocation. K1: Deletion. K2: Intercalary translocation and deletion. TL: Translocation line.
Fig 2.
Grouping of the 74 wheat-A. cristatum 2P translocated chromosomes detected in the M1BC1, F2 plants.
The P-genomic DNA signal was red (wheat DNA was stained blue by DAPI). A: Large alien segmental translocation containing the whole 2P chromosome long arm. B: Large alien segmental translocation containing the whole 2P chromosome short arm. C: Small alien segmental translocation containing the partial 2P chromosome long arm. D: Small alien segmental translocation containing the partial 2P chromosome short arm. E: Whole arm translocation containing the whole 2P chromosome long arm. F: Whole arm translocation containing the whole 2P chromosome short arm. G: Intercalary translocation.
Table 3.
Effect of 60Co-γ irradiation and gametocidal chromosome 2C on translocation breakpoints and types.
Table 4.
Identification of the translocated wheat chromosomes.
Fig 3.
Dual-color FISH/GISH identification of the homoeologous group of partially translocated wheat chromosomes.
A1-R1: GISH patterns of wheat-A. cristatum translocation lines. Total genomic DNA of A. cristatum was labelled with digoxigenin-11-dUTP and visualized with red fluorescence. Chromosomes were counterstained with DAPI and visualized with blue fluorescence. A2-R2: FISH patterns of wheat-A. cristatum translocation lines. A2-N2, P2-R2: The repetitive sequence clone pHvG39 was labelled with biotin-16-dUTP and visualized with green fluorescence; O2: The repetitive sequence clone pSc119.2 was labelled with biotin-16-dUTP and visualized with green fluorescence. The repetitive sequence clone pAs1 was labelled with digoxigenin-11-dUTP and visualized with red fluorescence. Chromosomes were counterstained with DAPI and visualized with blue fluorescence. Arrows point to the translocated chromosomes. A. 2P-23; B. 2P-35; C. 2P-40; D. 2P-43; E. 2P-48; F. 2P-55; G. 2P-80; H. 2P-116; I. 2P-122; J. 2P-167; K. 2P-173; L. 2P-187; M. 2P-190; N. 2P-205; O. 2P-213; P. 2P-269; Q. 2P-355; R. 2P-367.