Fig 1.
Protein differential displays of AFS from ES and normal pregnant.
(A) 2D-DIGE gel stain by silver stain: Of these 12 protein spots, 5 were up-regulated and 7 were under-regulated in the ES subjects compared with the normal controls. Red arrow (spot 2, 7–9 and 10): up-regulated; Blue arrow (spot 1, 3–6 and 11–12):under-regulated. (B) 2D-DIGE images of AFS from women carrying ES and chromosomally normal fetuses: Spot 1: Vitamin D binding protein (VDBP); Spot 2: Apolipoprotein A1 (ApoA1); Spot 3–5: Alpha-1-antitrypsin(A1AT); Spot 6: Insulin-like growth factor-binding protein 1(IGFBP-1); Spot 7: Transthyretin (TTR).
Table 1.
Significant differentially expressed proteins identified in the amniotic fluid of women with Edwards syndrome fetuses.
Fig 2.
Western blot analysis of IGFBP-1(IGFBP-1 = IBP-1 = insulin growth factor binding protein 1, TTR, A1AT, and ApoA1in normal and ES AFS.
(A) Equal protein amounts of AFS from the women carrying normal and ES fetuses were separated by gel electrophoresis and immunoblotted with the appropriate dilution of antibody. One membrane out of the four independent replicates is shown. The nonspecific bindings of human IgG was used as internal loading controls. (B) Quantification of protein content of IGFBP-1, TTR, A1AT, and ApoA1 using scanning densitometry. Each bar represents the mean (SD) of four independent experiments. Dark striped bars, normal; light bars, ES; **p <0.003, * p <0.02
Fig 3.
Biological network analysis of the differentially expressed proteins using MetaCore mapping tools.
Five differentially expressed proteins in the pregnancies with Edwards syndrome (Trisomy 18) fetuses (as shown in red solid circles indicating over-expressed levels; blue solid circles indicating under-expressed levels) were found to interact with proteins in this network. Some proteins are shown as gene symbols: IBP = IGFBP-1 = insulin growth factor binding protein 1; APOA1 = apolipoprotein A1; VDB = vitamin D binding protein.
Table 2.
Top 10 biological processes and diseases that may be associated with the differentially expressed proteins in the women with Edwards syndrome (Trisomy 18) fetuses.