Fig 1.
Hydrolytic pathway from ginsenosides Rb1, Rb2, and Rc to C-K via C-Mc, ginsenoside Rd, and F2 by SS-bgly supplemented with CS-abf.
Fig 2.
Effect of supplementation of CS-abf to SS-bgly on the conversion of ginsenoside Rc to C-K.
Data represent the means of three experiments and error bars represent standard deviation.
Fig 3.
Production of C-K from PPD-type ginsenosides in RGE by SS-bgly supplemented with CS-abf.
Ginsenosides Rb1 (open circle), Rb2 (open square), Rc (open triangle), and Rd (open diamond) were converted to C-K (filled circle) via F2 (open inverted triangle) and C-Mc (filled triangle). Data represent the means of three experiments and error bars represent standard deviation.
Fig 4.
Effect of supplementation of CS-abf to SS-bgly on the concentration of C-Mc in RGE.
Data represent the means of three experiments and error bars represent standard deviation.
Fig 5.
Effects of (A) pH and (B) temperature on the production of C-K from PPD-type ginsenosides in RGE by SS-bgly supplemented with CS-abf.
Data represent the means of three experiments and error bars represent standard deviation.
Fig 6.
Effect of the concentration of (A) SS-bgly supplemented with CS-abf and (B) PPD-type ginsenosides in RGE.
Filled circle and open inverted triangle represent concentration of C-K and conversion yield, respectively. Data represent the means of three experiments and error bars represent standard deviation.
Fig 7.
C-K production from PPD-type ginsenosides in RGE.
C-K (filled circle) was produced from ginsenosides Rb1 (open circle), Rb2 (open square), Rc (open triangle), and Rd (open diamond) in RGE via F2 (open inverted triangle) and C-Mc (filled triangle) by (A) SS-bgly. (B) SS-bgly supplemented with CS-abf under the optimized unit ratio of the two enzymes. Data represent the means of three experiments and error bars represent standard deviation.
Table 1.
Production of C-K from PPD-type ginsenosides in ginseng extracts.
Table 2.
Contents of PPD- and PPT-type ginsenosides in 10% (w/v) RGE and GRE.