Fig 1.
Electropherograms of samples 1–8 obtained from 3’ direct triplet primed PCR followed by capillary electrophoresis.
(A)- (H): samples 1 to 8. Peaks in the eletropherogram indicate the number of CGG repeats in each individual.
Table 1.
CGG repeat sizes and methylation status elicited from capillary electrophoresis, methylation specific PCR and Southern hybridization analysis for the nineteen sub-samples.
Fig 2.
Agarose gel electrophoresis of methylation specific PCR.
(A), (B) and (C): samples 1 to 8. (D), (E) and (F): samples 9 to19. Top panel, non methylated PCR (Non Met PCR). Middle panel, methylated PCR (Met PCR). Bottom panel, methylated triplet primed PCR (mTP-PCR). L: l kb DNA molecular weight marker (Promega), N: Negative control (without genomic DNA).
Fig 3.
Autoradiogram of Southern hybridization carried on EcoRI and NruI digested genomic DNA hybridized with StB12.3 digoxigenin labeled probe.
(A): samples 1 to 8. (B): samples 9 to19. L-DNA molecular weight marker II dig labeled (Roche).