Fig 1.
Classification of the significant differentially expressed genes under cold conditions into functional categories (Rockhopper P<0.05 and Q<0.05).
Green and red bars represent up- and down-regulated genes, respectively.
Fig 2.
Functional enrichment of differentially expressed genes using Blast2GO software.
A. Down-regulated genes. B. Up-regulated genes. P<0.05 using Fisher’s Test.
Fig 3.
Organization of genes coding for ethanol oxidation in P. extremaustralis and other Pseudomonas species.
Arrows indicate the direction of gene transcription and the relative size of each open reading frame (ORF).
Fig 4.
Growth of the wild type (WT), ppqB and exaA strains.
A. Growth at 30°C. B. Growth at 8°C. Values represent the mean ± standard deviations (SD) from three independent cultures.
Fig 5.
Relationship between ethanol metabolism and the ß-oxidation pathway.
Solid black lines and names represent genes without differences in their expression. Solid red lines and names represent down-regulated genes and green solid lines and names indicate up-regulated functions under cold conditions. Dashed lines show probable relationships.
Fig 6.
Estimation of alcohol dehydrogenase activity using p-rosaniline plate assay in wild type (WT), ppqB and pqqB/pBBR1MCS5 pqqBCDE strains.
Activity was considered as positive in magenta colonies, while white colonies were considered as negative. A. Plates incubated at 30°C during 24 h. B. Plates were incubated at 8°C during 7 days. C. Determination of p-rosaniline index at 30°C. D. Determination of p-rosaniline index at 8°C. The asterisk (*) denotes significant differences (P<0.05) between strains (indicating by connector lines) using the Student's t test.