Fig 1.
Morphological and viability analysis of Puccinia psidii uredospores.
P. psidii uredospores from E. grandis (A) and P. guajava (B) exhibit similar morphology and germination viability, respectively (C and D).The arrows indicate the fungal germ tube in both uredospore populations, 24 hours after inoculation in water-agar medium. Light microscopy images of PpEucalyptus and PpGuava uredospores are shown at 100 X (A and B) and 200 X (C and D) magnification. Scale bar: 20 μm in A and B, 50 μm in C and D.
Fig 2.
Eucalyptus infection by guava and eucalyptus rust.
Symptoms induced by inoculation of P. psidii uredospores from PpEucalyptus (A) on E. grandis variety D901. This clone is rust susceptible when grown under field conditions. The white arrows indicate the fungal pustules. The leaves are shown 15 days after inoculation.The PpGuava populations (B) and control (C) did not show typical rust symptoms.
Fig 3.
Proteins identified from P. psidii uredospore populations.
The proteins exclusively found in PpGuava (right) and PpEucalyptus (left) and common to both populations (center). Of the common proteins, 25 and 120 whose abundance were increased in PpEucalyptus and PpGuava, respectively.
Table 1.
Identification of proteins that were differentially represented in the P. psidii uredospore populations from E. grandis (PpEucalyptus) and Psidium guajava (PpGuava).
Fig 4.
Gene ontology of biological process terms in the proteomic analysis.
Bar graph represents the ratio of % composition of term in the proteomic data.
Fig 5.
Protein profiles in PpGuava and PpEucalyptus and the correlation with their physiological variability.
While proteins correlated to fungal virulence and stress response had the abundance increased in PpGuava, proteins related to biogenesis, protein folding and translation had the abundance increased in PpEucalyptus.