Table 1.
Pathological characteristics of interest for 135 NSCLC samples.
Fig 1.
A- Percentage of samples suitable for gene expression arrays (RNA integrity number [RIN] of ≥7) at different ex-vivo procurement times (time from surgical removal to cryopreservation) for ≤1 hours, 1–2 hours, 3–4 hours, 5–9 hours and ≥10 hours. B- Relation between ex-vivo procurement times (<1 and 1–2 hours, from excision to cryopreservation) and suitability for gene expression arrays (RNA integrity number [RIN] of ≥7) and time periods 2010, 2011, and 2012.
Fig 2.
Integrity of total RNA isolated from banked fresh-frozen lung cancer samples.
Representative electropherograms (Agilent 2100 Bioanalyzer) show RNA integrity measured by RNA integrity number (RIN) of 12 representative samples (bottom).
Table 2.
Correlation of RNA quality (RIN>7) and ex-vivo ischemia time, surgical time and pathological variables.
Multivariate logistic regression analysis on 135 NSCLC samples.
Fig 3.
A- Cq expression levels of housekeeping genes in randomly selected 28 samples representative of all the time categories. B- Gel electrophoresis of 28 genomic DNA amplified with multiplex PCR amplifying at different numbers of base paires (100 bp, 200 bp, 300 bp, 400 bp, 600 bp).
Table 3.
Correlation of PDX engraftment and ex-vivo ischemia time, surgical time and pathological variables.
Multivariate logistic regression analysis on 63 NSCLC cases.