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Fig 1.

Picture of two cultures of Leptospira sp. strain Manara.

Two cultures of Leptospira sp. strain Manara in Fletcher medium supplemented with 3% (left tube) and 1% (right tube) seawater after 48 h of incubation at 28°C. The dinger rings corresponding to Leptospira sp. growth are indicated with red arrows.

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Table 1.

Growth results of strain Manara in EMJH and Fletcher media diluted with seawater.

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Table 2.

Growth results of subcultures of strain Manara in Fletcher media diluted with seawater after 8 days.

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Fig 2.

Immunofluorescence of strain Manara.

Immunofluorescence of strain Manara isolated from a kidney sample of a Southern right whale (Eubalaena australis) 40X.

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Fig 3.

Neighbor-joining analysis of the sequence 16S rRNA of strain Manara.

Phylogenetic analysis based of 16S rRNA including 19 representative species of Leptospira spp. The corresponding sequence of Leptonema illini strain DSM 21528 was used as outgroup. The dendrogram was constructed using Neighbor-joining. Bootstrap values are displayed as percentages.

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