Fig 1.
Distribution of the length of transcript assembly unigenes.
Table 1.
Summary of short-read data from hydrangea produced by Illumina sequencing.
Table 2.
The length distribution of assembled contigs, transcripts and unigenes.
Table 3.
Annotation of Unigenes were searched against from NR, SwissProt, GO, KOG, KEGG by BLAST.
Fig 2.
The overview of differential expression between no Al and Al stress in roots and leaves in hydrangea.
(A) Boxplots of the DEGs in the four samples; (B) Sequencing saturation level analysis of DEGs in the four samples; (C) Density distribution of the differentially expressed genes (DEGs) in the four samples; (D) heat map of the DEGs in roots and leaves between Al(-) and Al (+).
Fig 3.
Histogram of KOG classification of differential expressed unigenes in roots (A) and leaves (B) under Al stress in hydrangea.
Fig 4.
Gene Ontology classifications of differential expressed unigenes in roots (A) and leaves (B) under Al stress in hydrangea.
Fig 5.
KEGG enrichment analysis of differential expressed unigenes in roots (A) and leaves (B) under Al stress in hydrangea.
Fig 6.
Validation of the expression data from RNA-seq analysis via quantitative real-time RT-PCR (qPCR) analysis.
Twenty genes exhibiting diverse expression profiles in the RNA-seq data were chosen for qPCR analysis. Average value of each RNA-seq expression data was plotted against that from qPCR and fit into a linear regression. Both x- and y-axes were shown in log2 scale.
Table 4.
Comparison of expression profiling between STOP/ART-regulated genes in rice or Arabidopsis and corresponding genes in hydrangea roots.
Fig 7.
Expression analysis of differential expression unigenes in root and leaves under Al stress in hydrangea by quantitative real-time RT-PCR (qPCR).
Eighteen genes in roots: ABC (c166032.graph_c0), ABC transporter B family member 12; AtpJ (c176296.graph_c0), ATP synthase subunit J; CHA (c149908.graph_c2), Chitinase-like protein; COX11 (c220486.graph_c0), Cytochrome c oxidase assembly protein COX11; CWP(c193058.graph_c1), Cell wall protein DAN4; DDI (c201184.graph_c0), DNA damage-inducible protein 1; GAT (c211725.graph_c0), Glucose/galactose transporter; HMT (c205800.graph_ c0),Heavy metal tolerance protein; MAD (c86593.graph_c0), Malate dehydrogenase; MAP(c119898.graph_c0), malate carrier protein; MFS(c219821. graph_c0), Major facilitator superfamily; MHF(c185758. graph_c0), Metal homeostasis factor ATX1; MMA1 (c152377.graph_c0), Mitochondrial metalloendopeptidase; MMT1(c142806.Graph_c0), Mitochondrial metal transporter 1; PCD6 (c127753.Graph _c0), Programmedcell death protein 6; MRA (c211816.graph_c0), Multidrug resistance- associated protein 1; MYB (c156038. graph_c0), Myb-like protein; SUP(c161922.graph_c0), Subtilisin-like proteinase Spm1. Two genes in leaves: AMT (c208330. graph_ c1), Aluminum-activated malate transporter; POT (c86493.graph_c0), Potassium transporter 1. The asterisks indicate significant differences between root (+Al) and root (-Al), or leaves (+Al) and leaves (-Al), as determined by Student’s t test (*P<0.05, **P < 0.01, ***P < 0.001).
Table 5.
Al-induced transporter genes in hydrangea roots.
Table 6.
Al-induced transporter genes in Hydrangea leaves.
Table 7.
Classification of SSR repeats in the hydrangea transcriptome assembly.
Fig 8.
SNP distribution of different variation types in hydrangea.