Fig 1.
A. Waterfall plot for the 5-fluorouracil IC50 (μM) values. B. Waterfall plot for oxaliplatin IC50 (μM) values. C. Waterfall plot for BEZ235 IC50 (nM) values. D. Unsupervised hierarchical clustering for the IC50 values for 5-FU, L-OHP and BEZ235 using Pearson’s Correlation with complete linkage.
Fig 2.
Karyogram for chromosome 1 to 22 showing the most frequent gains and losses for the 15 CRC cell lines
Table 1.
Summary of the regions of copy number gains and the genes significantly overexpressed in those regions (* after Bonferroni correction).
Table 2.
Summary of the regions having copy number losses.
Fig 3.
Hierarchical clustering using the genomic segmentations of the 15 CRC cell lines.
Fig 4.
A. A heatmap depicting the SAM analysis for genes differentially expressed between 5-FU sensitive and less sensitive CRC cell lines; B. A heatmap depicting the SAM analysis for genes differentially expressed between L-OHP sensitive and less sensitive CRC cell lines; C. A heatmap depicting the SAM analysis for genes differentially expressed between BEZ235 sensitive and less sensitive CRC cell lines.
Fig 5.
Venn diagram showing differentially expressed genes with respect to treatment response to a) 5-FU, b) L-OHP, and c) BEZ235B.
Fig 6.
Unsupervised hierarchical clustering for the 47 candidate genes annotated according to response to therapy.
Fig 7.
Unsupervised hierarchical clustering of RPPA protein expression data using Euclidian distance with average linkage.
Fig 8.
A. Box plots showing significant differences in protein expression between 5-FU sensitive and less sensitive cell lines (Mann Whitney U test) B. Box plots showing significant differences in protein expression between L-OHP sensitive and less sensitive cell lines (Mann Whitney U test).
Fig 9.
Spearman’s correlation network using Bonferroni Correction (p = 0.05) and circular network layout (http://www.tmanavigator.org/).
Abbreviations: N—nucleus, C—cytoplasm.