Fig 1.
Line drawings of Phasmarhabditis huizhouensis sp. nov.
(A) Female reproductive system (in: intestine; oo: oocytes; gsc: aggregated sperm cells; dus: distal part of uteri filled with sperms; em: embryos; vu: vulva). (B) Female tail (ph: phasmid). (C) Anterior region of female (ex: excretory pore). (D) Anterior region of male. (E) Spicules and gubernaculum (1: spicule in dorsal view; 2: spicule in ventral view; 3: gubernaculum from sublateral view). (F) Lateral view of male tail (1–9: nine pairs of genital papillae; ph: phasmid). (G) Ventral view of male tail with an open peloderan bursa supported by 9 pairs of genital papillae and 1 terminal pair of phasmids.
Fig 2.
Micrographs of Phasmarhabditis huizhouensis sp. nov.
(A) Overview of female (left) and male (right) adults. (B) Pharyngeal region of an adult female. (C) Pharyngeal region of an adult male. (D) Anterior end (stoma) of a female. (E) Anterior end (stoma) of a male. (F) Vulva. (G) Female tail. (H) Lateral view of male tail. (I) and (J) Ventral view of male tails showing ten pairs of bursal papillae [nine pairs of genital papillae (1–9) and one terminal pair of phasmids (ph) (I)]. (K) Ventral view of the male caudal bursa. (L) Spicules (sp) and gubernaculum (gu).
Fig 3.
Scanning electron micrographs of Phasmarhabditis huizhouensis sp. nov.
(A) and (B) Head of females (LS: labial sensilla; a: amphid; CS: cephalic sensilla). (C) Head of male. (D) Anterior region of male showing excretory pore (exp). (E) Magnification of the excretory pore. (F) and (G) Lateral fields of females (1–4: 4 incisures compose the three middle ridges/bands). (H) Lateral field of male. (I) and (J) Ventral and lateral views of female tails (ph: phasmid). (K) Magnification of female phasmid. (L) and (M) Male caudal region with an open bursa as well as its 9 pairs of genital papillae (1–9) (cp: cloacal papillae). [(B), (F), (I), (J) and (K) were captured by the Hitachi_S-3400N scanning electron microscope, others by the Hitachi_S-450].
Table 1.
Morphometrics of Phasmarhabditis huizhouensis sp. nov. [20].
All measurements are in μm and shown in the form: mean ± s.d. (range).
Fig 4.
Bayesian tree inferred from SSU rDNA sequences.
Posterior probability values exceeding 50% are given on appropriate clades. GenBank and strain ID associated with DNA sequences are shown on the left and right of the species name respectively.
Fig 5.
Bayesian tree inferred from LSU D2-D3 rDNA sequences.
Posterior probability values exceeding 50% are given on appropriate clades. GenBank and strain ID associated with DNA sequences are shown on the left and right of the species name respectively.