Table 1.
Primer information for 6 selected candidate reference genes.
Fig 1.
Expression levels of reference genes.
(A) Expression of reference genes for 5 V. parahaemolyticus strains. (B) Expression of reference genes for all culture temperatures. Gene expression levels are represented by average Ct values. Each bar represents the mean Ct values from three independent experiments. Error bars indicate standard deviations.
Table 2.
Descriptive statistics of reference gene expression in 5 V. parahaemolyticus strains for all growth temperatures by BestKeeper.
Fig 2.
Analysis of reference gene stability by NormFinder, Delta CT, and GeNorm.
For NormFinder and Delta CT, lower standard deviations (SD) values indicate more stable gene expression. For GeNorm, lower M values indicate more stable gene expression.
Fig 3.
Analysis of reference gene stability by RefFinder.
Ranking of candidate reference genes according to stability values produced by GeNorm, NormFinder, BestKeeper, and Delta CT, and the recommended comprehensive ranking calculated by RefFinder.
Table 3.
Ranking of candidate reference genes according to the stability value of each V. parahaemolyticus strain for all growth temperatures.
The stability measurements were produced by GeNorm, NormFinder, BestKeeper, and Delta CT, and the recommended comprehensive ranking was calculated by RefFinder.
Table 4.
Ranking of candidate reference genes according the stability values of 5 V. parahaemolyticus strains at each culture temperatures for.
The stability measurements were produced by GeNorm, NormFinder, BestKeeper, and Delta CT, and the recommended comprehensive ranking was calculated by RefFinder.