Fig 1.
(A) The pubic symphysis fibrocartilage samples; (B) Embedding in the optimum cutting temperature (OCT) compound; (C) A single click representing a precession of 20 μm. 100 20 μm was achieved by 5 clicks for each layer.
Fig 2.
(A–C) The size of “book”-shape cartilage scaffolds before decellularization. (D–F) The size of decellularized “book” cartilage scaffolds.
Fig 3.
(A–C) Hematoxylin and eosin (H&E) staining of “book” cartilage scaffolds before decellularization (magnification: A, ×50; B, ×100; and C, ×400); (E–G) H&E staining of decellularized “book” cartilage scaffolds (magnification: E, ×50; F ×100; and G, ×400); (D, I, J) Masson’s trichrome staining of “book” cartilage scaffolds before decellularization (magnification: D, ×50; I, ×100; and J, ×400); (H, M, N) Masson’s trichrome staining of decellularized “book” cartilage scaffolds (magnification: H, ×50; M, ×100; and N, ×400); (K, L) 4ʹ,6-diamidino-2-phenylindole (DAPI) staining of “book” cartilage scaffolds before decellularization (magnification: K, ×100; L, ×400); (O, P) DAPI staining of decellularized “book” cartilage scaffolds (magnification: O, ×100; P, ×400 magnification).
Fig 4.
(A, B) Scanning electron microscopy (SEM) of “book” cartilage scaffolds before decellularization (A ×500 SE; B ×5000 SE); (C, D) SEM of decellularized “book” cartilage scaffolds (C ×500 SE; D ×5000 SE).
Fig 5.
Quantification of DNA in fibrocartilage.
DNA was measured at 0.97±0.29 μg/mg (fibrocartilage dry weight) before decellularization and 0.08±0.02 μg/mg after decellularization.
Fig 6.
Quantification of collagen and GAG.
The quantity of collagen was 228.68±72.32 μg/mg (dry weight) before decellularization and 158.70±50.17 μg/mg after decellularization. The quantity of GAG was 55.43±6.11 μg/mg (dry weight) before decellularization and 28.41±4.83 μg/mg after decellularization.