Fig 1.
Viral dosage and duration of adenovirus-mediated gene delivery after a subconjunctival injection in the rats.
(A) Bioluminescence images of rat eyes at day 1, 2, 7, 14, 28, 35 and 64 after a subconjunctival injection of Ad-Luci (1x109 and 1x1010 GC/eye). (B) Luciferase activity was measured in unit of photons by bioluminescence analysis. Data are presented as the means ± SEM (n = 5).
Fig 2.
Bio-distribution of adenovirus after a subconjunctival or intravenous injection in the rats.
(A) Bioluminescence images of rat at day 1, 2, 7, 14, 28, 35 and 64 after a subconjunctival or intravenous injection of Ad-Luci (1x1010 GC/eye). (B) Luciferase activity was measured in unit of photons by bioluminescence analysis. Data are presented as the means ± SEM (n = 5).
Fig 3.
Local ocular distribution of adenovirus after a subconjunctival injection in the rats.
Histological sections of the rat eyes injected with Ad-LacZ (1x1010 GC/eye) in the subconjunctiva. The blue staining (arrows) in the image indicate the expression of β-galactosidase. V: vitreous; S: sclera. Scale bar = 200 μm (left) and 50 μm (right).
Table 1.
Evaluation of ocular distribution and local inflammatory response after a subconjunctival injection of adenoviral vector in the rats.
Fig 4.
Effect of liver function in the rats received a single injection of adenoviral vector via different administration routes.
(A) Serum level of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) were measured at 14 days after an intravenous or subconjunctival injection of Ad-Luci (1x1010 GC/eye). Data are presented as the means ± SEM (***: p<0.0001, n = 6). One-way ANOVA followed by a Tukey’s test. (B) Sections of liver tissue were stained for TUNEL (green) and DAPI (Blue) 14 days after injection of adenovirus through different administration route. Scale bar = 200 μm.
Fig 5.
The serum level of IL-1β and CINC-1 in the rats received a single injection of adenoviral vector via different administration routes.
Serum level of interleukin-1β (IL-1β; A) and cytokine-induced neutrophil chemoattractant factor-1 (CINC-1; B) were measured at 2 hours and 7 days after an intravenous or subconjunctival injection of Ad-Luci (1x1010 GC/eye). Data are presented as the means ± SEM (*: p<0.05, ***: p<0.0001, n = 6). One-way ANOVA followed by a Tukey’s test.
Table 2.
Analysis of retinal function by electroretinography (ERG).