Fig 1.
Expression of fibro-slime genes in F. succinogenes S85 cellulose and glucose cultures.
The cellulose cultures showed an increased expression for 8 out of 10 fibro-slime proteins relative to the glucose cultures. RPKM = Reads Per Kilobase per Million mapped reads.
Fig 2.
Expression of pilin transcripts in F. succinogenes S85 cellulose and glucose cultures.
There is a higher expression of pilin proteins in the cellulose cultures than the glucose cultures for 3 out of the 4 pilin proteins observed. RPKM = Reads Per Kilobase per Million mapped reads.
Fig 3.
Difference in expression between cellulose and glucose cultures for transporters, secretion systems, effluxes, and exporters genes.
All type II and III secretion systems had higher expression in the cellulose cultures than the glucose cultures suggesting they may have a role in secreting the carbohydrate active enzymes into the extracellular medium. RPKM = Reads Per Kilobase per Million mapped reads.
Fig 4.
(A) Average number of carbohydrate active enzymes (CAZymes) and (B) average abundance of CAZymes observed from a given fraction. (C) Percent of CAZymes to total proteins and (D) percent CAZymes abundance to total protein abundance.
The number and percentages of CAZymes and abundance of CAZymes was largest for the extracellular medium fraction. EM = extracellular medium, OM = outer membrane, PE = periplasm.
Fig 5.
Comparison of the number of identified proteins and their overlap observed in the outer membrane, periplasm and extracellular medium fractions.
Table 1.
Carbohydrate active enzymes (CAZymes) and fibro-slime proteins observed from proteomes extracted from extracellular medium, outer membrane, and periplasm fractions.
Fig 6.
Average abundance of fibro-slime proteins identified from each fractionation.
The average abundance for all fibro-slime proteins was the highest in the extracellular medium.
Fig 7.
TEM images of F. succinogenes S85 harvested during mid-exponential growth on cellulose (A, B), cellulose stationary growth (C, D), glucose mid-exponential growth (E) and glucose stationary growth (F).
Grooves in the cellulose were observed in the cellulose mid-exponential and stationary phases. Vesicles were present in only the cellulose stationary phase (arrows in D). Scale bars are 1 μm (A,C) and 0.5 μm (B-F). Vesicles are indicated by arrows. Grooves are indicated by curves.