Table 1.
An overview of small RNA categories.
Fig 1.
Size distribution of small RNA sequences in library CK, A and M.
CK: control; A: aphid infestation treatment; M: mock puncture treatment; nt: nucleotide.
Fig 2.
The expression profiles of conserved microRNAs (miRNAs).
CK: control; A: aphid infestation treatment; M: mock puncture treatment. A: number of miRNAs detected in library CK, A and M; B: number of differentially expressed miRNAs identified in CK-VS-A, CK-VS-M and M-VS-A comparisons.
Fig 3.
Heat map of differential expression and cluster analysis of conserved microRNAs (miRNAs).
MiRNAs who have similar pattern of differential expression in different library comparisons are clustered together. Red: up-regulation; green: down-regulation; gray: miRNA has no expression in at least one library. CK: control; A: aphid infestation treatment; M: mock puncture treatment. CK-VS-A: comparison between library CK and A; CK-VS-M: comparison between library CK and M; M-VS-A: comparison between library M and A.
Fig 4.
Target validation of miR160a and miR393a in chrysanthemum.
A: cleavage site in the target of miR160a; B: cleavage site in the target of miR393a. The 5’ end of the cleavage product is indicated by arrow with the frequency of clones. Vertical dash: Watson-Crick pairing; Circle: G:U wobble pairing.
Fig 5.
Gene Ontology (GO) functional classification of targets of conserved microRNAs (miRNAs).
Targets were annotated in three categories: biological process (blue), cellular component (red) and molecular function (green). CK: control; A: aphid infestation treatment; M: mock puncture treatment. A: comparison between library CK and A (CK-VS-A); B: comparison between library CK and M (CK-VS-M); C: comparison between library M and A (M-VS-A).
Fig 6.
The expression profiles of potential novel microRNAs (miRNAs).
CK: control; A: aphid infestation treatment; M: mock puncture treatment. A: number of miRNAs detected in library CK, A and M; B: number of differentially expressed miRNAs identified in CK-VS-A, CK-VS-M and M-VS-A comparisons.
Fig 7.
Validation of the expression of microRNAs (miRNAs) from deep sequencing in leaf tissues of chrysanthemum.
Correlation of fold change was analyzed by deep sequencing (x axis) with data obtained using qRT-PCR (y axis).