Table 1.
Differential susceptibility of International Space Station isolates a.
Table 2.
Biofilm percent composition at 72 h based on differential plating.
Fig 1.
Effect of species absence on exoenzyme production from model communities.
Static bacterial assemblages (‘Mix’ and ‘species absence’, where a minus symbol in front of the species abbreviation denotes removal) or single species cultures (‘species alone’) were assayed for (A) short-chain lipase activity using NPB, (B) long-chain lipase activity using NPP, (C) choline-specific phospholipase C activity using NPPC, and (D) protease activity using FITC-casein. Results are compiled from three separate experiments each with three biological replicates and error bars represent SEM. Samples all compared to ‘Mix’ (first bar) using ANOVA with Dunnett’s post-test. Statistical symbols: * = p < 0.05; ** = p < 0.01; # = p <0.001. Abbreviations: Ralstonia pickettii (Rp), Cupriavidus metallidurans (Cm), Chryseobacterium gleum (Cg), Ralstonia insidiosa (Ri), Sphingomonas sanguinis (Ss), Burkholderia multivorans (Bm), Phyllobacterium myrsianacearum (Pm), Sphingomonas paucimobilis (Sp), and Burkholderia cepacia (Bc).
Fig 2.
Liposomes were not significantly lysed by exposure to community exoproducts for four hours.
This data is compiled from three independent experiments each with three biological replicates and error bars represent SD. Data was analyzed by one-way ANOVA and a Dunnett’s post-test was used to compare all groups to heat-killed biofilm supernatant ‘HK sup’ (first bar). Statistical symbols: NS = not significant; *** = p < 0.001. Abbreviations: Mix = supernatant from mixed culture; Triton = Triton X-100 treatment; PlcH = treatment with 1 ng/ul purified PlcHR (CITE); Pa Sup = supernatant from Pseudomonas aeruginosa PAO1 as a postitive lipolytic control.
Fig 3.
Effect of species absence on exoenzyme production from model communities using a comparison to predicted production based on single species cultures.
Expected values for exoenzyme production were compared to measured value for (A) short-chain lipase activity, (B) long-chain lipase activity, (C) choline-specific phospholipase C activity, and (D) protease activity (from Fig 1). Error bars represent SEM. Samples all compared to ‘Mix’ (first bar) using ANOVA with Dunnett’s post-test. Statistical symbols: * = p < 0.05; ** = p < 0.01. Abbreviations: Ralstonia pickettii (Rp), Cupriavidus metallidurans (Cm), Chryseobacterium gleum (Cg), Ralstonia insidiosa (Ri), Sphingomonas sanguinis (Ss), Burkholderia multivorans (Bm), Phyllobacterium myrsianacearum (Pm), Sphingomonas paucimobilis (Sp), and Burkholderia cepacia (Bc). The Mix-K and–Cg-K denote predictions based on the final population proportion as measured in Table 2.