Fig 1.
CPE caused by Mangshi virus (DH13M041) on C6/36 cells 72 h postinoculation (100×).
Fig 2.
Electrophoretic migration patterns of the dsRNA of Mangshi virus (DH13M041) as determined by polyacrylamide gel electrophoresis.
1: DH13M127-6 (BAV); 2:C6/36 Cells control; 3: DH13C233-5 (BAV); 4:DH13M041 (Mangshi virus).
Fig 3.
PCR Identification of DH13M041 virus in the culture supernatant of C6/36 cells using primers derived from segment 12 of BAV by 1% agarose gel electrophoresis (AGE).
1: DH13M127-6; 2: DH13C233-5; 3: DH13M041. 4: Negative control.
Table 1.
Lengths of dsRNA segments 1 to 12, encoded putative proteins, 5′ and 3′ NCRs of the Mangshi virus (DH13M041) genome.
Table 2.
The identity of amino acid between the Mangshi virus (DH13M041) and other Seadornaviruses.
Fig 4.
Alignment of the sequence of guanylyltransferases of members of the family Reoviridae in the Mangshi virus (DH13M041) VP3 with other Seadornaviruses.
(a) Alignments of sequences of guanylyltransferases in the vicinity of the motif Kx(I/V/L)S is shown in bold characters. Similar sequences are shaded. (b) Alignments of sequences of guanylyltransferases in the vicinity of the two histidine residues (bold) involved in the guanylyltransferase activity in BAV, KDV, and LNV. Similar sequences are shaded.
Fig 5.
Phylogenetic analysis of complete amino acid sequences of corresponding viral proteins (VP, see Table 1) of Mangshi virus (strain DH13M041) using representative members of the species Balaton virus, Banna virus (BAV), Kadipiro virus (KDV) and Liao ning virus (LNV) in the genus Seadornavirus.
(A) RNA-dependent RNA polymerase (VP1); (B) T2 layer of core/subcore (VP2); (C) protein kinase (VP8); (D) outer-coat attachment protein (VP9) of Mangshi virus.