Table 1.
Morphological and genetic characterization of 37 putative strains of Clavibacter michiganensis subsp. nebraskensis collected from Goss’s leaf blight symptomatic and asymptomatic maize leaves compared with two strains of Clavibacter michiganensis subsp. michiganensis.
Fig 1.
Examples of colony morphology after 5 days growth on nutrient broth yeast extract (NBY) medium of 37 putative isolates of Clavibacter michiganensis subsp. nebraskensis.
A small mucoid orange colony; B large mucoid orange colony; and C large fluidal yellow colony.
Fig 2.
Mean aggressiveness (n = 12 plants) of 28 Clavibacter michiganensis subsp. nebraskensis strains estimated from foliar blight severity on maize hybrid DKC 55–09.
Plants were inoculated at the V3–V4 crop developmental stage and foliar blight severity was rated as the proportion of leaf area affected six days after inoculation. Bars represent standard errors of the mean. *Strain 91-R was included as a reference for pathogenicity in this experiment.
Fig 3.
Representative RFLP patterns obtained after restriction of rpoD and recA with BstU1 for 37 putative isolates of Clavibacter michiganensis subsp. nebraskensis and two strains of Clavibacter michiganensis subsp. michiganensis.
Roman numerals correspond to pattern types observed. Molecular size marker: GeneRuler 100 bp DNA Ladder.
Table 2.
Nucleotide polymorphisms in the sequences of putative virulence factors found in the genome of Clavibacter michiganensis subsp. nebraskensis strains GIL1 and HF4. PCR primers flanking each locus were used in sequencing reactions to obtain the complete sequence of each gene.