Fig 1.
Parallel and antiparallel-stranded DNA duplexes depending on pH value (A) and chemical structure of the Watson-Crick and reverse Watson-Crick as well as Hoogsteen base pairs (B).
Table 1.
Summary of biophysical properties of D1-D18 duplexes at various buffers pH values.a
Fig 2.
CD spectra of D1-D18 duplexes at pH 5.0 (black line) and 7.0 (red line).
Fig 3.
Time-resolved fluorescence lifetime analysis of the DNA/RNA-LNA (D4) duplex at pH 7.0.
Top panel—donor fluorescence decay in the presence of acceptor (black line) and the instrument response function (IRF, red line); bottom panel—the weighted residuals.
Fig 4.
The fluorescence lifetime and normalized fluorescence intensity of selected D1, D3, D7 and D9 duplexes at pH 7.0.
Black line indicates the S1 control probe for D1-D6 duplexes and the S2 control probe for D7-D12 duplexes. Red and blue lines indicate respective duplexes.
Fig 5.
Normalized fluorescence intensity of D1-D18 duplexes at pH 7.0 (blue line) and pH 5.0 (red line).
Black line indicates fluorescence of control probe S1 for D1-D6 duplexes, control probe S2 for D7-D12 duplexes as well as control probe S3 for D13-D18 duplexes.