Fig 1.
Hematoxylin and eosin staining of representative white perch ovary tissue sections.
Ovary tissues were sampled at four key time points across one reproductive year during (A) pre-vitellogenesis (PreVG), (B) early-vitellogenesis (EVG), (C) mid-vitellogenesis (MVG), and (D) post-vitellogenesis (PostVG). Scale bar is 500 microns.
Table 1.
Sexually mature female white perch sampling statistics.
Fig 2.
Protein cleavage-isotope dilution mass spectrometry (PC-IDMS) tandem mass spectrometry results for the three white perch vitellogenins (VtgAa, VtgAb, and VtgC).
Results from protein cleavage-isotope dilution mass spectrometry (PC-IDMS) tandem mass spectrometry for the three white perch vitellogenins (VtgAa, VtgAb, and VtgC) in female liver, plasma, and ovary tissues sampled across one reproductive year during pre-vitellogenesis (PreVG), early-vitellogenesis (EVG), mid-vitellogenesis (MVG), and post-vitellogenesis (PostVG) across 3 biological replicates. The mean ± SD is shown. “N.Q.” indicates that the native peptide was not quantifiable. Levels not connected by the same letter are significantly different at α = 0.05.
Fig 3.
White perch vitellogenin Aa, Ab, and C western blotting.
Results of western blotting for the three white perch vitellogenins in female liver, plasma, and ovary tissues sampled across one reproductive year during pre-vitellogenesis (PreVG), early-vitellogenesis (EVG), mid-vitellogenesis (MVG), and post-vitellogenesis (PostVG) pooled from three individuals at each time point.
Fig 4.
White perch LR8 and Lrp13 western blotting.
Results of western blotting for the two white perch vitellogenin receptors in female liver, plasma, and ovary tissues sampled across one reproductive year during pre-vitellogenesis (PreVG), early-vitellogenesis (EVG), mid-vitellogenesis (MVG), and post-vitellogenesis (PostVG) across three biological replicates.
Fig 5.
Confocal microscopy of anti-VtgC coupled to DyLight633.
Confocal microscopy images of immunohistochemistry of mature female white perch ovary tissues across one reproductive year stained with anti-VtgC coupled to DyLight633: (A) pre-vitellogenic (PreVG), (B) early-vitellogenic (EVG), (C) mid-vitellogenic (MVG), and (D) post-vitellogenic (PostVG) ovary sections.
Table 2.
VtgC affinity purification coupled to tandem mass spectrometry.
Fig 6.
Vitellogenin composition in white perch and striped bass egg yolk.
In white perch (M. americana), yolk proteins derived from VtgC are minor components of the total egg yolk (< 5%), whereas in striped bass (M. saxatilis) they are major components of the egg yolk (~ 25%). [Williams et al., 2014 (J Exp Zool Part A); Schilling et al., 2014 (J Proteome Res)].
Fig 7.
Average survival duration of food-restricted white perch and striped bass larvae.
Dashed boxes indicate approximate time of hatching (~2 days) and onset of first feeding (~4 days in white perch, ~8 days in striped bass). [Mansuetti, 1964; Eldridge, et al., 1981; North & Houde, 2003].