Fig 1.
ONC201 effects on proliferation and cell death in tumor and normal cells.
(A) Cell viability assays following ONC201 treatment at indicated concentrations for 72 hours (n = 3). Doxorubicin (Doxo) was used at 1 μg/mL as a positive control in normal fibroblasts. (B) Cell cycle analysis of tumor and normal cells following treatment with ONC201 (5 μM) or doxorubicin (Doxo, 1μg/mL) for 72 hours (n = 3). (C) Cell viability assay in MRC5 cells following a 72 hour treatment with ONC201 (5 μM) or DMSO and the indicated recovery period in complete drug-free media after this treatment (n = 3). Time points are given as time following removal on ONC201 after 72 hour treatment. (D) Cell viability of tumor and normal cells following treatment with ONC201 (5 μM, n = 3) for 72 hours.
Fig 2.
Effect of ONC201 on surface TRAIL and DR5 In tumor and normal cells.
(A) Surface TRAIL and DR5 expression in HCT116 (tumor) and HFF (normal) cells following treatment with ONC201 (5 μM, 72 hr, n = 3). IgG was included as an isotype negative control. Doxorubicin (Doxo) is shown as a positive control (1 μg/mL). (B) Surface TRAIL and DR5 in normal fibroblasts following treatment with ONC201 (5 μM) or doxorubicin (1 μg/mL, 72 hr, n = 3). Values (y-axis) is expressed as mean fluorescence intensity.
Table 1.
Cohorts used in GLP toxicology studies with ONC201.