Table 1.
Multivariate analysis of 345 SNPs associated with KD susceptibility in patients and cohort controls (p ≤ 0.05).
Table 2.
Multivariate analysis of 345 SNPs associated with CAL formation in KD patients (p ≤ 0.05).
Table 3.
Best fit results using multifactor dimensionality reduction analysis of one-, two-, and three-locus models for KD susceptibility in patients and cohort controls.
Table 4.
Best fit results using multifactor dimensionality reduction analysis of one-, two-, and three-locus models for CAL formation in KD patients.
Fig 1.
PDE2A (rs341058) and CYFIP2 (rs767007) gene-gene interaction in a two-way mode of MDR analysis.
The interaction of PDE2A and CYFIP2 was significantly associated with increased KD risk in logistic regression of our MDR results from KD patients (n = 226) and cohort controls (n = 575), with an odds ratio of 3.54 (95% CI: 2.17–5.78) and a p-value of 4.14 x 10−7. (A) MDR classified the nine interactive items of allele combinations into high- or low-risk KD groups, which were significantly different in our further analysis using the Chi-square test (p = 9.71 x 10–7). (B).
Fig 2.
LOC100133214 (rs2517892) and IL2RA (rs3118470) gene-gene interaction in a 2-way mode of MDR analysis.
The interaction of LOC100133214 and IL2RA was significantly associated with a higher risk of CAL formation using logistic regression of our MDR results from KD patients with CAL (n = 73) and KD patients without CAL (n = 153), with an odds ratio of 5.35 (95% CI: 2.33–12.25) and a p-value of 7.46 x 10−5. (A) MDR classified the nine interactive items into high- or low-risk CAL groups, which were significantly different in our further analysis using the Chi-square test (p = 3.36 x 10−6). (B).
Fig 3.
Comparison cytokines levels between KD patients with high-risk genotypes and low-risk genotypes.
KD patients possessing the high-risk (KD risk: 1) PDE2A (rs341058) and CYFIP2 (rs767007) genotypes of KD susceptibility (n = 49) presented with significantly lower plasma levels of TGF-β1 (9489 ± 1605 vs. 16133 ± 3015) compared to KD patients in the low-risk group (KD risk: 0, n = 24), with an odds ratio of 0.59 (p = 0.036). (A) KD patients possessing the high-risk LOC100133214 (rs2517892) and IL2RA (rs3118470) genotypes of CAL formation (CAL risk: 1, n = 35) presented with significantly elevated plasma levels of IL-2 (14.1 ± 1.6 vs. 9.6 ± 1.2) compared to KD patients in the low-risk group (CAL risk: 0, n = 38), with an odds ratio of 1.47 (p = 0.028). (B) KD patients possessing the high-risk LOC100133214 (rs2517892) and IL2RA (rs3118470) genotypes of CAL formation (CAL risk: 1, n = 35) presented with significantly elevated plasma levels of IL-6 (51.0 ± 14.3 vs. 18.4 ± 3.7) compared to KD patients in the low-risk group (CAL risk: 0, n = 38), with an odds ratio of 2.77 (p = 0.033). (C) KD patients possessing the high-risk LOC100133214 (rs2517892) and IL2RA (rs3118470) genotypes of CAL formation (CAL risk: 1, n = 35) presented with significantly elevated plasma levels of IFN-γ (119.2 ± 15.2 vs. 81.8 ± 10.1) compared to KD patients in the low-risk group (CAL risk: 0, n = 38), with an odds ratio of 1.46 (p = 0.041). (D).