The anti-proliferative effects of HDIs and CDDP in breast cancer cell lines.
(A) The anti-proliferative effects of VPA, SAHA, and CDDP on T47D, MCF7, MDA-MB-231 breast cancer cell lines after 96 h treatment with various concentration (0.1–1000 μg/ml) of active substances. The cell viability was measured by the MTT assay. (B) The anti-proliferative effects of VPA, SAHA, and CDDP on T47D breast cancer cell line. The cell viability was measured by the MTT assay. T47D cells were exposed to concomitant or sequential VPA and CDDP treatment using different ratios of the IC50 (1/2 IC50, IC50, 3/2 IC50). VPA+CDDP—valproic acid and cisplatin concomitant, CDDP→VPA—cisplatin (1h) followed by valproic acid, VPA→CDDP—valproic acid (1h) followed by cisplatin. Statistical analysis was performed using one-way ANOVA test (*** p<0.001). The results are means ± standard deviation (SD). (C) Examination of cell proliferation by MTT assay. T47D cells were exposed to concomitant or sequential SAHA and CDDP treatment using different ratios of the IC50 (1/2 IC50, IC50, 3/2 IC50). SAHA+CDDP—vorinostat and cisplatin concomitant, CDDP→SAHA cisplatin (1h) followed by vorinostat, SAHA→CDDP—vorinostat (1h) followed by cisplatin. The results are presented as mean ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA test (*** p<0.001).
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