Fig 1.
1A. Growth and resting cyst production in the cross culture. 1B. Percentage of cells in the different phases of the cell cycle during the four L:D periods studied in the cross culture.
Fig 2.
Comparisons between clonal and cross cultures.
Percentage of cells in each cell cycle stage in the clonal culture (A) vs. the cross culture (B) during two L:D periods as shown by a trend line and the standard error (gray-shaded area). The dark bars along the bottom indicate the dark period. (C). Box-and-whisker plots comparing cell areas in the cell phases of clonal vs. cross culture.
Table 1.
Estimated growth parameters during sexual induction (cross culture H5×H7, phosphate-limited medium) during days 3–9 post-synchronization (DPS).
Table 2.
Estimated growth parameters for exponential clonal growth (culture H7, replete medium) during days PS2-3.
Fig 3.
IFC and cell sorting pictures.
IFC images show cell and nuclear morphologies in cells with different DNA contents as seen using bright field microscopy and after blue (488 nm, nuclei in orange) and violet (405 nm, nuclei in red) laser excitation respectively. To the right of IFC images are shown sorted cells with the same DNA content as the cells shown by the IFC pictures (A, B, D + subscript). All cells have the nuclei stained with IP. 2-clusters were additionally stained with calcofluor (in blue) in sorted cells.
Fig 4.
High-power magnification (1000×) images of the mitotic process in the clonal and cross cultures.
DNA DAPI staining (blue) and in situ hybridization of the Dy547-labeled oligonucleotide (CCCTAAA)3 used to localize telomeres (red) in Alexandrium minutum cells. Scale bars = 10 μm
Fig 5.
Schematic diagram of the processes that may take place in A. minutum cells during the cell cycle.