Fig 1.
The studied influencing factors related to excellent pig cloning.
A, donor cells derived from excellent Landrace or Large white breeding boars, B, 101–150, 151–200 or 201–250 cloned embryos transferred per surrogate, C, Day 1–5 of surrogate estrus, D, ovulation statuses including preovulation, periovulation and postovulation, and E, transfer position change or follicle puncture. Major factors including donor cell type, number of cloned embryos transferred per surrogate, surrogate estrus stage, ovulation status and embryo transfer manners were systematically investigated during excellent pig cloning.
Table 1.
Effect of donor cell types on in vivo development of cloned embryos.
Table 2.
Effect of transferred cloned embryo number per surrogate on in vivo development of cloned embryos.
Table 3.
Effect of surrogate estrus stage on in vivo development of cloned embryos.
Fig 2.
The percentage of surrogate ovulation statuses on Day 1–5 of estrus.
The proportions of preovulation, periovulation and postovulation on Day 1–5 of estrus. Preovulation and periovulation mainly occurred on Day 1 and Day 2, respectively, and all the surrogates were under postovulation on Day 4 and Day 5 of estrus. The number of surrogates detected was on the top of column chart. a-cValues for a given status with different superscripts differ significantly (P<0.05).
Fig 3.
Ovulation statuses of surrogate gilts when cloned embryos transferred.
A, preovulation, follicles large developed but not ovulated; B, periovulation, follicles partly ovulated; and C, postovulation, follicles all ovulated. Surrogate gilts during periovulation were suitable for pig cloning.
Table 4.
Effect of surrogate ovulation status on in vivo development of cloned embryos.
Table 5.
Effect of transfer manners on in vivo development of cloned embryos.