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Table 1.

Primer and probe sequences for the EBOV targets used in the Xpert® Ebola Assay.

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Fig 1.

Linearity of the Xpert® Ebola Assay and Altona RealStar® Ebolavirus RT-PCR Kit 1.0.

Serial dilutions of inactivated EBOV Yambuku-Mayinga was spiked into EDTA-whole blood and tested in triplicate. Linear regression lines are shown for the Xpert® Ebola nucleoprotein (NP) and glycoprotein (GP) targets (NP: y = -3.44x+46.11, R2 = 0.98; GP: y = -3.87x+51.45, R2 = 0.87), and Altona RealStar® Ebolavirus (y = -4.51x+50.17, R2 = 0.93).

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Table 2.

Range finding analysis of the Xpert® Ebola Assay, Altona RealStar® Ebolavirus RT-PCR Kit 1.0, and BioFire Defense LLC FilmArray Biothreat-E Test® using Inactivated EBOV Yambuku-Mayinga.

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Table 3.

Limit of Detection of the Xpert® Ebola Assay and Altona RealStar® Ebolavirus RT-PCR Kit 1.0 using Inactivated EBOV Yambuku-Mayinga.

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Table 4.

Numbers of positive replicates per level in the Xpert® Ebola Assay for EBOV Makona-C07, and for EBOV Yambuku-Mayinga RNA, both in EDTA-whole blood.

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Table 5.

Analytical Reactivity and Specificity (Inclusivity and Exclusivity).

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Table 5 Expand